FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341041
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29540.22027663583738302No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23370.17426760255652138No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATTTTTTTGGGTT20060.1495852848645194No Hit
GTCTACAAACTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA16540.12333701952438442No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14310.10670814687992389No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13970.10417280306866085No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13850.10327797584115625No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13780.10275599329177855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC7550.037.2490432
GGTATCA18100.037.155251
GTGGTAT7700.036.9853741
GTATCAA39100.036.593331
TATCAAC42700.033.0915832
TCAACGC44350.031.622554
ATCAACG44350.031.5417043
CAACGCA44850.030.9653515
AACGCAG45900.030.4086786
ACGCAGA49250.028.3423847
TTACGCT500.00739884627.8526749
CGCAGAG50850.027.5854828
AGAGTAC50850.027.58445211
CAGAGTA52600.026.7971210
GCAGAGT54650.025.6046129
AGTACGG38400.025.00590913
GAGTACG39400.024.45919212
GTACGGG44550.021.55150414
ATACCGA800.003002293921.4336366
TACGGGG23850.020.99954615
Produced by FastQC (version 0.11.5)