FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35728
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1250.34986565158978955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGTG206.555843E-572.5279961
AGAGAGG206.908148E-571.7679756
GCGGAGC150.002046183271.34077552
TAGTTGG150.002095030970.9186447
GCTAGTT150.00213223370.605345
GGCTAGT150.00213223370.605344
AAACGAT150.002157306870.3979443
GGGGCTA150.002182599970.19179542
TCAAACG150.002208113269.9868641
GTCAAAC150.002246799469.6816940
TGTCAAA150.002246799469.6816939
TTGTCAA150.002299164769.2789138
ATTGTGA150.00244786468.1948832
TTATTGT150.00251777467.7132830
AAAATTG150.002603618467.1442622
TGCCATT200.00581262554.81059665
GAGTGCT200.00591874954.5610863
GGAGTGC200.00595444254.47841362
AGGGAGT200.006098819454.15022760
AGAGGGA200.006135317554.06879858
Produced by FastQC (version 0.11.5)