FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26150
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT530.20267686424474185No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.10325047801147226No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCTC200.006727284852.7842742
GGTTTCT200.0068355352.5722941
GTCCAGC200.00830079150.0592731
CTTGAAC200.00830079150.0592733
TGAACCA307.300627E-444.4971355
GGTATCA705.657057E-1042.907951
GTATCAA1900.040.3987121
CTACATC350.001558602138.14041
TATCAAC2200.036.4067462
ATCAACG2350.034.082913
TACGGGC502.2028387E-433.3728515
AACGCAG2450.032.691776
CAACGCA2450.032.691775
TCAACGC2550.031.4097394
ACGCAGA2650.030.2244667
AGAGTAC2700.029.66475511
CAGAGTA2750.029.12539710
GCAGAGT2750.029.1253979
CGCAGAG2900.027.618918
AGTACGG1850.027.05906913
Produced by FastQC (version 0.11.5)