FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26150
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1350.5162523900573613No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.11089866156787764No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT280.10707456978967496No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGAG100.007430444142.1788670
TGTGCGT150.002637368466.875726
GTGGGTC200.00823718550.15678822
ATGCCCA200.00823718550.15678821
AACGCAG2600.034.723936
GTATCAA2750.034.045821
AGAGTAC2700.033.4378611
CAGAGTA2750.032.829910
TATCAAC2900.032.284832
GCAGAGT2800.032.243659
CGCAGAG2800.032.243658
ACGCAGA2900.031.13187
ATCAACG3150.029.722543
CAACGCA3150.029.722545
TCAACGC3350.027.9480634
GAGTACG2650.025.2361212
AGTACGG2700.024.76878413
AGTACTT951.2751516E-524.63842413
GAGTACT951.2751516E-524.63842412
TACGGGT851.7202176E-423.60319315
Produced by FastQC (version 0.11.5)