FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851741
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17070.2004130363572964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31150.039.7246441
ATCAACG36050.034.272783
TCAACGC36250.034.079694
CAACGCA36850.033.524795
GGTATCA13950.033.1719061
TATCAAC37300.033.141742
AACGCAG38100.032.42496
AGAGTAC39400.031.1813311
ACGCAGA40050.030.7607127
GTGGTAT5350.030.7537691
CAGAGTA39950.030.75204810
CGCAGAG41350.029.7936298
GCAGAGT42550.029.033819
TGGTATC6000.027.3947262
AGTACTT15100.025.83594313
GAGTACG32250.025.78534512
AGTACGG32350.025.7056413
GAGTACT15450.025.69365712
ACGGGAT3750.024.64511716
ACGGTAG700.00139379824.45670119
Produced by FastQC (version 0.11.5)