FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77899
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.107831936225112No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTATC150.002525537567.7379617
TTCATCT200.00666352753.01675852
GCTATTT200.007868650.8361212
TGTATCA200.00788862850.8034718
CCATTAA200.00788862850.803471
GACAGGT306.872606E-445.1586381
GTGGTAT451.1070036E-437.6322021
GTATCAA4450.036.532831
GAGCCTT400.002181160735.70345359
CTATAGT400.002773057234.028
ACGGGGG400.002826112633.8689816
TATCAAC4950.032.8426482
GCCTTGT552.6083164E-432.52374661
GGTATCA2300.032.396411
GTTGGAG602.9756274E-431.79811768
CCTAGGT450.00413638631.33333448
ACGGGTT553.5985687E-430.7899823
AACGCAG5450.030.4901756
TTACACC450.00503085830.105764
ATCAACG5400.030.1057573
Produced by FastQC (version 0.11.5)