FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77899
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1610.20667787776479798No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGTAC150.002473344868.0954228
CCTACCC200.00784502350.874763
TTACCAA200.00784502350.8747617
GTGCGTC200.00784502350.874763
GAGCCTT350.001177469440.47752854
GTGGTAT702.478373E-838.786631
GTATCAA4350.037.4491651
GGTATCA2100.033.9383051
GGAGCCT450.004054542631.46142253
TATCAAC5200.030.6553062
ATCAACG5200.029.3508243
TACGGGT704.2752697E-529.07129115
CAACGCA5300.028.7970335
TCAACGC5300.028.7970334
TGGTATC953.6449455E-728.561272
CGGGCTA605.940744E-428.26375417
GTCTTGG500.007057621628.08936348
AACGCAG5500.027.7498686
CACTGTG500.00835893327.1332045
ACGGGGA500.00835893327.13320416
Produced by FastQC (version 0.11.5)