FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011098
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACTACTGTGCGAGAGATCCCTCACCGGATCGGGGTGGCTACGATTG97380.48421310149977775No Hit
GTAGTAGACAGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGTAGATGCA97120.4829202753918506No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT75000.37293060805589784No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT56500.28094105806877634No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT52360.2603552885040908No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC52350.2603055644230167No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT46790.23265897534580612No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC41510.2064046605386709No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA41500.20635493645759678No Hit
GTCTCCAGCCACCCTGTCTTTATCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGCAG40530.2015317005934072No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40270.20023887448548006No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT39060.1942222606755116No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38780.19282998640543625No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG37400.1859680632172077No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCCCAGGGCCGAAAGTG37090.18442661670391No Hit
GTCTATTACTGTCACCAGCGTGACACCTGGCCTCAGGGCACTTTCGGCCC36550.18174151632590754No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36410.18104537919086985No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35610.1770674527049403No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA35470.1763713155699026No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34360.17085194257067532No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG34020.16916132381415525No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA33850.16831601443589522No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32780.1629955377609644No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCGCAGCTCCTTAGCCTGGTACCAACAGAGACCTGGCCAGGCTCCCAGGCT32120.1597137484100725No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGGAGTTATTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGG32090.15956457616685013No Hit
GTACCAACAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGC31040.15434354765406758No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT30130.14981865627632268No Hit
GCCTAGAGCCTGAAGATTCTGCAGTCTATTACTGTCACCAGCGTGACACC30050.14942086362772972No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC29790.14812803751980264No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG29730.14782969303335788No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA29590.14713355589832022No Hit
GTAATAGACTGCAGAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCGCAGACCCACCGCCA29430.1463379706011343No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29410.14623852243898608No Hit
GTCTTTATCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCGCAGCTCCTTAGCCTGGTA28980.14410038695279892No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC28590.14216114779090824No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT28180.14012246046686933No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC27980.13912797884538694No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27550.1369898433591998No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27510.13679094703490333No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT27280.13564729317019855No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT27030.13440419114334556No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT26220.13037654057634188No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT26200.13027709241419363No Hit
CTATTACTGTCACCAGCGTGACACCTGGCCTCAGGGCACTTTCGGCCCTGGGACCAAAGTGGAGATCAAACGAA25980.129183162630563No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25750.12803950876585826No Hit
CTCCAGAGACAACGCCAAGAACTCACTGCATCTACAAATGAACAGCCTGAGAGCCGACGACACGGCTGTCTACTA25260.1256030287932264No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACTCATCCAATAACTCC25080.12470799533389222No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25060.124608547171744No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG24410.12137648190192621No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24370.12117758557762973No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24150.12008365579399909No Hit
CAGTAATAGACTGCAGAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCGCAGACCCACCGCCA23900.1188405537671461No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT23820.11844276111855315No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23650.1175974517402931No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA23590.11729910725384839No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG23540.1170504868484778No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA23430.11650352195666248No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23170.11521069584873536No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA23030.11451455871369769No Hit
GCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTC22810.11342062893006707No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22640.11257531955180702No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGGTAGATGGAAGTGAGAAATATTA22530.11202835465999171No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG22450.11163056201139875No Hit
GTCTGCGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT22430.11153111384925052No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA22420.11148138976817638No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC21900.10889573755232217No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCGGTGGGTCTGCGACAGACTTCACT21610.10745373920117268No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21290.10586256860680088No Hit
GAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGT21130.10506698330961495No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC20820.10352553679631724No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACTTTGGTCC20570.10228243476946425No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGTCC20480.10183491803979718No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC20250.10069126417509242No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20210.10049236785079592No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC20150.10019402336435121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)