FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080529
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12350.11429586804241255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31150.040.8850671
TATCAAC35200.035.769332
ATCAACG35750.034.724743
TCAACGC36000.034.577254
CAACGCA36300.034.1970255
GGTATCA12700.033.246831
AACGCAG37650.032.8797536
TGGTATC6550.032.4741482
ACGCAGA39950.030.9868037
GTGGTAT7250.030.7768551
CAGAGTA41050.030.32353410
AGAGTAC41150.030.08039511
CGCAGAG41350.029.9376728
GCAGAGT45250.027.5089749
AGTACGG34950.027.07746713
GAGTACG36100.026.2148912
ACGGGGC4450.023.12106916
GTACGGG40950.022.9436714
TACGGGT7000.022.04452715
GTATTAG3600.021.9317971
Produced by FastQC (version 0.11.5)