FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846469
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT45450.5369363792412953No Hit
ATATTATAGTGCTCCTCCTACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT44160.5216966008205852No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39140.46239141657875243No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC38530.4551850097286493No Hit
ATTTTATACAGCTCCAACAATAAGATGTACTTAGCTTGGTACCAGCAGAA38110.45022322140562737No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28330.3346844361695467No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26400.31188383744708903No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTACATCTTATTGTTGGAGCTGTATAAAATACT26150.3089303920167189No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24750.29239109760664594No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23270.2749067006588546No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22580.2667551912710329No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCATAGAGGTCAGTCTCAGGGAGA22290.2633291945718036No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTA21570.25482327173233754No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21430.2531693422913302No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC21290.2515154128503229No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19920.23533053189189446No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19590.23143198392380585No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGACAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTTTATA19330.22836040067622088No Hit
CTATTACACTGGGAGCACCTTCTACAACCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTAGACACGTCCAGCAA18150.2144201382448737No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17880.2112304171800739No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA17520.2069774557603409No Hit
CACCTGCACTGTCTCGGGTGGCACCCTCAGCAGTAGTTACTACTGGGGCT17370.2052053885021188No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17290.20426028596440038No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC16710.19740829256594156No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC16700.19729015474872677No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15840.1871303024682534No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15540.1835861679518092No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14770.17448955602626912No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14700.17366259130576547No Hit
GTTTATTACTGTCAGCAATATTATAGTGCTCCTCCTACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA14410.1702365946065361No Hit
GTGTATTACTGTGCGGGACATAAGGACCGGGAGCTACTAAGCTGGTTCGC14360.16964590552046205No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14110.1666924600900919No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT13910.16432970374579578No Hit
CTCCCTGGCTGTGTCTCTGGGCGACAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTTTATACAGCTCCAA13910.16432970374579578No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13660.16137625831542562No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13640.161139982680996No Hit
CCCTGGCTGTGTCTCTGGGCGACAGGGCCACCATCAACTGCAAGTCCAGC13350.1577139859817666No Hit
GTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATC13300.1571232968956926No Hit
GTCTCGGGTGGCACCCTCAGCAGTAGTTACTACTGGGGCTGGATCCGCCA13070.15440612709975202No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13070.15440612709975202No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12880.1521615085726707No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12630.14920806314230053No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12580.14861737405622652No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12440.14696344461521924No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12180.14389186136763427No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTGCTCCTCCTACTTT12030.14211979410941217No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT11920.1408202781200493No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA11900.14058400248561967No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11820.13963889994790124No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11750.13881193522739757No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTGCT11730.13857565959296797No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11640.1375124192380347No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11570.13668545451753106No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11530.13621290324867183No Hit
GTCTCTGGGCGACAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTATTTTATACAGCTCCAACAATAAGATGTA11530.13621290324867183No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11460.1353859385281682No Hit
GTATAAAATACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTGTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA11390.13455897380766454No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11240.13278690654944245No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11140.1316055283772944No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGACAGGGCCACCATCAACTG11110.13125111492564998No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT10850.128179531678065No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG10780.12735256695756134No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA10760.12711629132313174No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10720.1266437400542725No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10220.12073684919353218No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10140.11979174665581374No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9950.11754712812873241No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT9910.11707457685987319No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG9820.11601133650493993No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9810.11589319868772513No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC9800.11577506087051032No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTGCTCCTC9780.1155387852360807No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9720.11482995833279187No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCATAGAGGTCAGTCTCAGGGA9690.11447554488114745No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGACAGGG9520.11246720198849573No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9490.11211278853685133No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA9330.11022258346141442No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA9330.11022258346141442No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC9310.1099863078269848No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9310.1099863078269848No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9170.10833237838597752No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA8950.10573334640725178No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG8770.10360686569738525No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8710.10289803879409641No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8630.10195293625637797No Hit
CCCTGAGACTGACCTCTATGACCGCCGCAGACACGGCTGTGTATTACTGTGCGGGACATAAGGACCGGGAGCTA8570.10124410935308914No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8570.10124410935308914No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8560.10112597153587433No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGATGA8550.10100783371865953No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8520.1006534202670151No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8510.10053528244980028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATA150.00240173568.661333
ACGCTAA300.00249160268.0223170
AACGCTA302.1935726E-456.98210569
TACGGAT7000.050.5308570
GTTATTA554.406255E-449.47076470
CTTATAC1350.045.774221
GGTATCA5750.044.1820761
GTATCAA15350.042.0466771
TATACAC1700.040.3890153
CTAGTCT350.001382212339.22577717
GTGGTAT1800.038.1451871
CGTATGC1200.037.50176241
CCCACGA1750.037.2666919
TCGTATG1151.8189894E-1236.0919940
ATCAACG18350.035.920913
TATCAAC18350.035.733822
TCAACGC18600.035.2535324
ACGAGAC1950.035.21093822
CAACGCA18850.035.1502275
AACGCAG19100.034.69226
Produced by FastQC (version 0.11.5)