FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224446
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54980.44901939326029894No Hit
CCTCATATATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTT43470.35501769779965797No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA40290.32904676890610124No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGACT35570.29049872350434397No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33550.27400146678579534No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31250.2552174616112103No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGACTTTGCAGTTTATTACTGTCAGCAGTATGATAAATGG30990.253094052330605No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29890.24411039768189044No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27900.22785814972648855No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27230.2223862873495442No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGGCCATTTATCATACTG26950.22009953889350775No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26840.2192011734286363No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT25900.2115242321833711No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25730.2101358491922061No Hit
GTCTGAAGACTTTGCAGTTTATTACTGTCAGCAGTATGATAAATGGCCTC24600.20090718578034475No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24270.19821208938573037No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23710.19363859247365747No Hit
GTCTCCGGCCACCCTGTCTGTGTCTCCAGGAGAAAGAGCCATCCTCTTCTGCAGGGCCAGTCAGAGTGTTAGCAG23460.1915968527807678No Hit
CATATATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA23360.1907801569036119No Hit
GTAATACACAGCCGTATCTGCGGCGGTCACAGAACTCAACTTCAGGGAGA22270.1818781718426129No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC21980.1795097537988609No Hit
GTATGATAAATGGCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT21440.17509959606221917No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20950.1710977862641554No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19240.15713228676478994No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT19080.15582557336134056No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19070.155743903773625No Hit
ATATATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGCCAAGTTGCTGCTAACACTCTGACTGGC19070.155743903773625No Hit
GTCTCTGGTGACTCCATCAGTAGGAACAGTTACTACTGGGGCTGGATCCG18370.15002703263353387No Hit
GTCTGTGTCTCCAGGAGAAAGAGCCATCCTCTTCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTGGCCTGGTA17970.14676024912491037No Hit
GTGTATTACTGTGTGAGACCGGGCGCCAGCACCTGGTACGATAAGCCTAT17910.14627023159861685No Hit
CCATATATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGC17770.14512685737059863No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCA17150.14006334293223222No Hit
GTTTATTACTGTCAGCAGTATGATAAATGGCCTCCGTGGACGTTCGGCCA17140.13998167334451664No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC16850.1376132553007646No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16770.1369598985990399No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16430.13418313261670994No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16040.13099801869580202No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16030.13091634910808642No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15940.13018132281864614No Hit
GTCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACC15740.1285479310643344No Hit
CACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAG15670.12797624395032528No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15670.12797624395032528No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15330.12519947796799533No Hit
ACCTACTACAGCCCGTCCCTCAAGAGTCGAGCCTCCATCTCCGTTGACAC15200.12413777332769269No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15150.12372942538911476No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14700.12005429394191332No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14580.11907425888932628No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14500.11842090218760158No Hit
CTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT14310.11686918002100542No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14280.11662417125785865No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14200.11597081455613396No Hit
CTGCTACTCTGGCTCCCAGATTCCACTGGAGAATTGGTGATGACGCAGTC14160.11564413620527161No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14120.11531745785440925No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13810.11278570063522607No Hit
CCAGTGGCCCTGGTGGATGCACCATATATGAGGAGCCTGGGAGCCTGGCC13740.11221401352121693No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13680.11172399599492341No Hit
CAGTAATACACAGCCGTATCTGCGGCGGTCACAGAACTCAACTTCAGGGA13660.11156065681949225No Hit
GGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGT13560.11074396094233638No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13500.11025394341604286No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12830.10478208103909852No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12800.10453707227595173No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT12630.10314868928478675No Hit
GTCAGAGTGTTAGCAGCAACTTGGCCTGGTACCAGCAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATATATGGTG12580.10274034134620882No Hit
GTCTCACACAGTAATACACAGCCGTATCTGCGGCGGTCACAGAACTCAAC12470.10184197588133735No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTC12390.10118861917961265No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12270.10020858412702562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCG2150.041.5312127
GGTATCA10500.041.4997981
CTAGCGC2250.041.2218528
AACGGAG6400.040.0216570
CTCGTAT1300.039.838439
GTATCAA25250.039.8141821
TGGTATC3400.039.356572
TCGTATG1350.038.39130440
GTGGTAT3550.037.6936151
TAGCGCT2400.037.2248329
CTTATAC2800.035.5362431
TATCAAC29100.034.3108522
AGCGCTC2650.033.7185730
CCCACGA2850.033.70753519
ATCAACG29950.033.338453
TATACAC3300.033.272493
TCAACGC30650.032.5783844
CGTATGC1600.032.43134741
AACGCAG31200.032.1166886
CAACGCA31200.032.115385
Produced by FastQC (version 0.11.5)