FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224446
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55360.4521228375934913No Hit
CCTCATATATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTT40880.33386527458132087No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA39390.32169650601169836No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGACT35580.29058039309205963No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34110.27857496369786827No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32360.2642827858476405No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGACTTTGCAGTTTATTACTGTCAGCAGTATGATAAATGG30280.24729551160279833No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28980.23667846519977198No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGGCCATTTATCATACTG28550.2331666729280017No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27500.22459136621786505No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25950.21193258012194902No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25070.20474565640297734No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT24480.1999271507277577No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23340.19061681772818076No Hit
GTCTGAAGACTTTGCAGTTTATTACTGTCAGCAGTATGATAAATGGCCTC23210.1895551130878781No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22860.18669667751783253No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22510.183838241947787No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21920.17901973627256734No Hit
CATATATGGTGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA21610.17648797905338415No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC20970.17126112543958658No Hit
GTAATACACAGCCGTATCTGCGGCGGTCACAGAACTCAACTTCAGGGAGA20890.17060776873786185No Hit
GTATGATAAATGGCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT20740.16938272492212805No Hit
GTCTCCGGCCACCCTGTCTGTGTCTCCAGGAGAAAGAGCCATCCTCTTCTGCAGGGCCAGTCAGAGTGTTAGCAG20530.1676676635801007No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20320.1659526022380734No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19480.15909235686996404No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18700.15272212902814825No Hit
GTCTCTGGTGACTCCATCAGTAGGAACAGTTACTACTGGGGCTGGATCCG18590.1518237635632768No Hit
ATATATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGCCAAGTTGCTGCTAACACTCTGACTGGC18540.15141541562469885No Hit
GTCTGTGTCTCCAGGAGAAAGAGCCATCCTCTTCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTGGCCTGGTA18450.15068038933525857No Hit
GTGTATTACTGTGTGAGACCGGGCGCCAGCACCTGGTACGATAAGCCTAT18350.1498636934581027No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17840.14569854448460773No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17790.14529019654602982No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17640.144065152730296No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17570.14349346561628687No Hit
GTTTATTACTGTCAGCAGTATGATAAATGGCCTCCGTGGACGTTCGGCCA17380.14194174344969074No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC16920.1381849424147737No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCA16900.13802160323934254No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT16900.13802160323934254No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16620.13573485478330607No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15990.13058967075722408No Hit
CTGCTACTCTGGCTCCCAGATTCCACTGGAGAATTGGTGATGACGCAGTC15480.12642452178372915No Hit
CCATATATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGC15460.12626118260829797No Hit
GTCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACC15390.12568949549428884No Hit
CACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAG15350.12536281714342648No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15040.12283105992424329No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14810.12095265940678479No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14390.11752253672273011No Hit
ACCTACTACAGCCCGTCCCTCAAGAGTCGAGCCTCCATCTCCGTTGACAC13900.11352072692466635No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13790.11262236145979487No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13690.111805665582639No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13650.11147898723177666No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13620.1112339784686299No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13530.11049895217918962No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13440.10976392588974931No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13370.1091922387757402No Hit
CTGGTGTCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT13340.10894723001259345No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13300.1086205516617311No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13150.10739550784599729No Hit
CAGTAATACACAGCCGTATCTGCGGCGGTCACAGAACTCAACTTCAGGGA12840.10486375062681409No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12330.10069860165331913No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)