FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326517
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35600.037.7971041
ATCAACG42050.032.1622963
TCAACGC42100.031.9638564
TATCAAC42900.031.6846682
CAACGCA42650.031.551665
AACGCAG43350.031.3581266
GGTATCA17550.029.6539421
AGAGTAC45700.028.3926711
ACGCAGA48050.028.2887067
CGCAGAG47600.028.1964918
CAGAGTA47500.027.4629610
GCAGAGT51450.026.0200029
AGTACGG40800.022.90792313
GAGTACG41200.022.85171112
GTGGTAT8000.022.2550891
CGAATTA953.2293494E-421.62274615
TGGTATC8350.020.9121972
GTATAAT1551.0807416E-619.8804761
CGCCATA900.00514125219.5324356
TGCTAGT900.00599245619.0214442
Produced by FastQC (version 0.11.5)