FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540273
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8200.15177512109618654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTC200.00756467551.3867842
GTATCAA15000.036.568821
ATCAACG17100.032.424763
GGTATCA7100.031.8689541
TCAACGC17350.031.7514574
TATCAAC17500.031.5172252
CAACGCA17850.030.862065
GTGGTAT3050.029.2250821
AACGCAG19150.028.7669876
CAGAGTA19450.028.49920310
AGAGTAC19450.028.3206611
ACGCAGA19900.027.8547467
CGAATTA500.00804517927.3758415
AGAGTCG1005.3838085E-727.3733065
CGCAGAG20450.026.9382788
GCAGAGT20800.026.6494929
CGAAATT1202.2372587E-623.24570748
CCGAAAT1352.421384E-723.21942747
AGTACGG18150.022.62047413
TGGTATC3800.022.5380612
Produced by FastQC (version 0.11.5)