FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794566
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41470.5219201425683958No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10150.12774269223701998No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGC150.002152022470.5880652
TACGGAT2000.060.82082770
TAGACCG306.779601E-445.33515
GTATCAA28750.040.60851
ACGATTG350.001253274540.01451547
GAACGAC350.001447985838.85620513
GGTATCA11000.038.3698161
TTCGACG1950.038.36736759
TCGACGT1900.037.5688260
TATCAAC31450.036.894162
GACGTGT1950.036.77313262
CTACGGA2400.035.73003469
CTAACGG400.002197870535.69038460
ATCAACG32750.035.4029053
TCAACGC32800.035.2452744
CGACGTG2050.034.90982461
CAACGCA33450.034.6620335
ACGTGTC2150.033.4233563
AACGCAG34550.033.361646
GTGGTAT4500.032.524951
Produced by FastQC (version 0.11.5)