FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103573
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17960.1627441048304009No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT12750.11553381606835252No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC12580.11399336518744116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11340.10275713523255825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC6600.044.228541
TCGTATG3550.042.86062640
CTCGTAT2900.042.7739739
TATACAC7000.041.701193
TTATACA7400.039.900492
CGTATGC4400.039.42518641
GTGGTAT11950.039.0278051
GTATCAA77700.037.0936361
GGTATCA36550.035.067371
CGAGACA8250.035.01580423
TACACAT8750.034.1278725
TCTCGTA3650.033.86659238
GCCGTCT4550.033.2902146
ACGAGAC8700.033.19560222
TCTCCGA8650.032.96486711
ATCTCCG8850.032.59895710
TATCAAC89250.032.5940362
AATCTCG4250.032.16943436
TCCGAGC8900.032.03743713
ACACATC9150.031.170576
Produced by FastQC (version 0.11.5)