FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103573
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61680.5589118254977242No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC13920.12613574271933076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11800.041.788731
GTATCAA82200.039.4619451
TATCAAC93400.034.4862332
GGTATCA37250.033.1394581
ATCAACG99600.032.2897453
TCAACGC99700.032.2529754
AACGCAG100650.032.1815076
CAACGCA100400.032.128195
ACGCAGA108450.029.8051597
CGCAGAG108900.029.651248
CAGAGTA110900.028.87487210
AGAGTAC110750.028.8534911
GCAGAGT113800.028.4628189
TGGTATC16800.027.731592
CGAATTA1157.494418E-826.21905915
GAGTACG68750.025.9685812
AGTACGG68400.025.95454813
TACGAAC700.001533417424.0598428
GCGAATT1400.001537328724.04669270
TACGGGA18500.023.7231315
Produced by FastQC (version 0.11.5)