FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247424
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51950.042.2143821
GGTATCA21300.038.5495761
TATCAAC58200.037.7383232
TCAACGC63150.035.0510834
ATCAACG63050.034.9981583
CAACGCA64250.034.450995
AACGCAG66450.033.2087636
GTGGTAT5900.032.4731181
TCGTATG2050.032.06544540
ACGCAGA71200.030.9439987
CGCAGAG72000.030.647698
AGAGTAC71550.030.02639211
CAGAGTA73000.029.52370310
GCAGAGT76400.028.9710399
ACGAGAC3100.027.59424622
AGTACGG46700.026.88344413
GAGTACG47100.026.72883612
CTCGTAT2500.026.2521639
CTTATAC3450.025.7835221
TCTCGTA2450.025.345238
Produced by FastQC (version 0.11.5)