FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451207
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG404.901395E-543.2665540
GTATCAA11050.034.7448651
GTACTAA601.40091615E-534.2795331
TATCAAC12450.030.8378122
ATCAACG12750.029.8433573
GTATTAG1151.8189894E-929.808291
TCAACGC12950.029.3824564
CAACGCA13200.028.8291655
AACGCAG13450.028.8030956
GGTATCA5350.028.192511
AGAGTAC13800.027.32729511
CGCAGAG14300.026.6115368
GTAACAT658.9787826E-426.3688723
ACGCAGA14500.026.244487
CAGAGTA14600.026.06472410
GCAGAGT15850.024.0091469
CGTATGC750.001945930323.09099441
ACGAGAC750.002058794722.86822722
TATACAC1202.5927966E-622.8530223
AATAGCG1102.6814916E-522.51793362
Produced by FastQC (version 0.11.5)