FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451207
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7560.16755059207858033No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTG350.005218425356.4339370
GTATCAA12750.043.5786361
ATCAACG14150.039.1973273
TATCAAC14500.038.2723962
CAACGCA14500.038.0024265
TCAACGC14500.038.0024264
AACGCAG14700.037.4853866
ACGCAGA16050.034.332417
CGCAGAG16450.033.4975788
CAGAGTA16000.033.15622710
AGAGTAC16200.032.74689511
GCAGAGT17550.031.3980149
GTATAAG802.885834E-630.010751
GGTATCA4800.029.296211
AGTACGG14450.028.18594213
TCCGGTC1103.947207E-828.0029168
GTGTTAG1353.274181E-1027.946521
GAGTACG14750.027.38062912
CGGGTAC659.043832E-426.33628317
TACGGGT3250.026.33044615
Produced by FastQC (version 0.11.5)