FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66281
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG1030.15539898311733377No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT990.14936407115161207No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT900.13578551922873824No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT890.13427679123730782No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA850.12824187927158612No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC800.120698239314434No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG750.11315459935728187No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA740.11164587136585145No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC730.11013714337442103No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT730.11013714337442103No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC720.10862841538299059No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG680.10259350341726889No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA670.10108477542583848No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGAT151.8564938E-4130.4869170
GCTCCGT150.001798942973.76745667
TGCTCCG150.001852372573.2259466
CCGTCTT308.195077E-658.44726647
TACGGGC252.7894953E-454.20377315
TACCTTG200.006555783653.22628855
ACTGCAC200.00691161152.52047745
TCCGTGA250.007379333551.64429569
CGGTCCT250.007379333551.64429569
GATGCCC200.007689243651.12471830
AGCATCG200.00782881850.8928619
TAAACAT200.00787575950.816045
GGATCAT200.00787575950.8160412
CCCCACT200.00787575950.8160413
ATTGCGG304.8645804E-448.4223365
GGTATCA1050.048.396231
TATTGCG304.9231795E-448.30508464
CTATTGC304.9823377E-448.18840463
CCGATGG305.3702533E-447.4623357
CTACCCC305.3702533E-447.4623357
Produced by FastQC (version 0.11.5)