FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3527681
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTCCAGGGAGA123110.3489828020163955No Hit
CTCCTGCACTGTCTCTGGTGACTCCACCAACAGTCCTATTTACTACTGGG96850.2745429646274706No Hit
GTCTATTACTGTGCGAGGGGCAGTCCTTCCTTCTTCGACCTCTATAGTAAGGAGTACTACTTTGACTACTGGGGC92130.2611630700168184No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC91020.2580165270045676No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT85530.24245389534938108No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT84400.23925065786844107No Hit
GTCTCTGGTGACTCCACCAACAGTCCTATTTACTACTGGGGCTGGATCCG82800.23471510037330473No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC75350.2135964107865762No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT71650.2031079340790735No Hit
CTATAGTAAGGAGTACTACTTTGACTACTGGGGCCAGGGAATCCTGGTCACCGTCGCCTCAGCCTCCACCAAGGG70960.20115197490929593No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG70080.19865741828697095No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG66200.1876586913612654No Hit
AGCTTACACTGGTACCAGCAGAAACCACATCAGTCTCCCAAGCTCCTCAT64340.18238610577316938No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62290.17657492273252598No Hit
TTACTATAGAGGTCGAAGAAGGAAGGACTGCCCCTCGCACAGTAATAGAC61250.17362681036068736No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA54910.15565466378620968No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC53970.1529900237578171No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT52120.14774578540406574No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC51190.14510949261001774No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA50900.1442874228140243No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA48880.1385612814764147No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT46260.13113430607812895No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC45810.12985868053262184No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA43890.12441601153845827No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA42030.11914342595036229No Hit
GTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCTCCTGCA40230.11404092376833394No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39790.11279364545717144No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC38920.11032743606919106No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38700.10970379691360982No Hit
GTATTACTGTCATCAGAGTAATAGTTTACCTCACACTTTTGGCCAGGGGA38030.10780453221252148No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37870.10735097646300786No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG37370.10593361474577774No Hit
CTCCACCAACAGTCCTATTTACTACTGGGGCTGGATCCGCCAGCCCCCAG37320.10579187857405474No Hit
CTATTATAGTGGGACCACCTTCTACAACCCGGCCCTCAAGAGTCGAGTCA36980.10482807260633828No Hit
GAGTAATAGTTTACCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT36750.10417608621641243No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA36610.103779224935588No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG36580.10369418323255418No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC35800.10148309895367524No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT35570.1008311125637494No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT35530.10071772362637096No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35370.10026416787685735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1850.048.6381240
TTCGGAT35350.048.2368770
CGCTAAT2150.046.5286432
GGTATCA26150.041.092241
TCGCTAA2600.039.79105431
GTGGTAT9700.039.056911
CGTATGC2250.038.4740441
GTATCAA58100.037.049371
TGGTATC10500.036.4091572
TATCAAC64450.033.3990442
GTTCGGA34550.033.31415669
ATCAACG68150.031.7373543
TCAACGC69100.031.2511774
CAACGCA70300.030.6687415
AACGCAG71400.030.0997776
CTCGCTA3700.028.88799330
ATCGTAT756.064881E-527.66779339
GCTAATC2650.027.34216533
CGCAGAG78800.027.3129838
TTCGGGG44500.027.30743870
Produced by FastQC (version 0.11.5)