FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3527681
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTCCAGGGAGA113920.3229316936537062No Hit
CTCCTGCACTGTCTCTGGTGACTCCACCAACAGTCCTATTTACTACTGGG91890.260482736392548No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT91880.26045438915820335No Hit
GTCTATTACTGTGCGAGGGGCAGTCCTTCCTTCTTCGACCTCTATAGTAAGGAGTACTACTTTGACTACTGGGGC90870.25759131848939854No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT82340.23341112759345303No Hit
GTCTCTGGTGACTCCACCAACAGTCCTATTTACTACTGGGGCTGGATCCG80820.22910234797307352No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC78160.2215619836374094No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT76390.21654452315841483No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC69700.19758022338187609No Hit
CTATAGTAAGGAGTACTACTTTGACTACTGGGGCCAGGGAATCCTGGTCACCGTCGCCTCAGCCTCCACCAAGGG68320.19366830504232102No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65940.18692166326830573No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG65160.18471057898942675No Hit
AGCTTACACTGGTACCAGCAGAAACCACATCAGTCTCCCAAGCTCCTCAT59150.16767389114832096No Hit
TTACTATAGAGGTCGAAGAAGGAAGGACTGCCCCTCGCACAGTAATAGAC58840.1667951268836383No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG56330.1596799710631432No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA55750.15803583147115627No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC50610.14346535301803082No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA50310.14261493598769276No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT49700.14088575469267203No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC49330.13983690702192175No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA46330.13133273671854115No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT45710.12957520818917584No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT44160.12518138686576252No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC44120.1250679979283841No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT43290.12271517747778214No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT42410.12022062085545718No Hit
GTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTCTCCTGCA42280.11985210680897733No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41470.11755598082706457No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41370.11727250848361855No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT41250.11693234167148334No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT39640.11236843694200241No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT39510.11199992289552259No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA39470.11188653395814417No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA38680.10964710244492062No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA38670.10961875521057601No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38140.10811635179031209No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA37630.10667064283873741No Hit
CTCCACCAACAGTCCTATTTACTACTGGGGCTGGATCCGCCAGCCCCCAG36760.10420443345075703No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG36450.10332566918607436No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG35900.10176657129712124No Hit
GTATTACTGTCATCAGAGTAATAGTTTACCTCACACTTTTGGCCAGGGGA35810.10151144618801983No Hit
CTATTATAGTGGGACCACCTTCTACAACCCGGCCCTCAAGAGTCGAGTCA35480.10057598745464796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAT31900.047.13467470
GGTATCA23900.041.7906531
GTGGTAT9750.040.2697831
TGGTATC9500.040.2207762
GTATCAA58050.037.852761
GTTCGGA32500.033.63213369
TATCAAC64950.033.4426962
ATCAACG68200.031.632323
TCAACGC69000.031.1093544
CAACGCA71000.030.3299375
AACGCAG72300.029.9749016
AGAGTAC78350.027.74772811
TTCGGGG43400.027.7160670
CGCAGAG79100.027.4846348
TGTTCGG37000.026.79077568
GCAGAGT82250.026.5993219
CAGCGCT18400.025.9867824
ACGCAGA85350.025.3917457
GTGTTCG37250.025.35949967
TCGTATA1951.8189894E-1225.14673261
Produced by FastQC (version 0.11.5)