FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1395487
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32240.23103045746753642No Hit
GTATAATGACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT29000.2078127564069031No Hit
CAGTAATACATGGCCGTGTCCGCGGCAGTCACAGAGGTCACCTTCAGGGAGAACTGGTTCTTAGACGTGTCTA23220.16639352426787207No Hit
GTTACAATACCGGGCTCGCGCACAGTAATACATGGCCGTGTCCGCGGCAGTCACAGAGGTCACCTTCAGGGAGAA23220.16639352426787207No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22550.16159233299916087No Hit
GTAATACATGGCCGTGTCCGCGGCAGTCACAGAGGTCACCTTCAGGGAGA21420.15349480145640912No Hit
GTCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTACTGATGGTGAGAGTGAACT20320.14561224862718178No Hit
GTATTGTAACAGTGCCAACTGCTATGGCGAAGTTGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGC20180.14460901463073464No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19480.13959284464849905No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGTAGCCTGCAGTCTGAAGATT18360.1315669726769221No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18070.12948884511285308No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17730.12705241969291006No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17180.12311114327829639No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17070.12232288799537366No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG16670.11945650514838188No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16610.11902654772133311No Hit
GTATTACTGTGCGCGAGCCCGGTATTGTAACAGTGCCAACTGCTATGGCGAAGTTGACTCCTGGGGCCAGGGAAC15980.11451199473732109No Hit
CACCTGCACTGTCTCTCGTGGCTCCATCAGCAGTGGTGGTTACTACTGGA15820.11336544159852438No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT15520.11121565446328056No Hit
GAGTTCACTCTCACCATCAGTAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATGACTGG15180.10877922904333756No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15140.10849259075863839No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15020.10763267590454086No Hit
ATGTATTACTGTGCGCGAGCCCGGTATTGTAACAGTGCCAACTGCTATGG14900.10677276105044331No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14770.10584118662517099No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14710.10541122919812222No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14530.10412135691697594No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGGCCAGTCATTATACTG14100.10103999535645979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23550.039.7786331
GGTATCA9900.037.9195481
TCGTATG1003.6925485E-1034.60936740
TATCAAC27250.034.2511062
TCAACGC28300.032.981494
ATCAACG28750.032.583893
CAACGCA28900.032.3002245
AACGCAG29800.031.3247186
CGTATGC1202.582965E-928.85464741
ACGCAGA33500.027.9647887
AGAGTAC33200.027.80152111
CGCAGAG33850.027.7773888
GCAGAGT35350.026.5987159
TACTGAA10600.026.57217870
CAGAGTA35800.026.07195910
ACCGTAC1101.1639295E-625.06394427
GAGTACG28000.023.24496812
AGTACGG27750.023.08208813
ACGAACT12150.022.37061755
GTGGTAT5400.022.3226721
Produced by FastQC (version 0.11.5)