FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1395487
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31770.22766245762232112No Hit
GTATAATGACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT28370.2032982034228911No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22630.16216560956855922No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21290.1525632270311368No Hit
GTTACAATACCGGGCTCGCGCACAGTAATACATGGCCGTGTCCGCGGCAGTCACAGAGGTCACCTTCAGGGAGAA21180.15177497174821405No Hit
GTCATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTACTGATGGTGAGAGTGAACT21150.15155999303468967No Hit
CAGTAATACATGGCCGTGTCCGCGGCAGTCACAGAGGTCACCTTCAGGGAGAACTGGTTCTTAGACGTGTCTA20570.14740373790655162No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19510.13980782336202344No Hit
GTATTGTAACAGTGCCAACTGCTATGGCGAAGTTGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGC18840.13500663209331223No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18470.13235522795984483No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGTAGCCTGCAGTCTGAAGATT18080.12956050468402786No Hit
GTAATACATGGCCGTGTCCGCGGCAGTCACAGAGGTCACCTTCAGGGAGA17930.12848561111640594No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17080.12239454756654845No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16890.12103301571422737No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16550.11859659029428435No Hit
CACCTGCACTGTCTCTCGTGGCTCCATCAGCAGTGGTGGTTACTACTGGA16010.11472697345084547No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGGAGGCCAGTCATTATACTG14850.10641446319456935No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14650.10498127177107346No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14610.10469463348637428No Hit
GAGTTCACTCTCACCATCAGTAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATGACTGG14230.10197156978173211No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14230.10197156978173211No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG14220.10189991021055732No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14160.10146995278350855No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14140.10132663364115896No Hit
GTATTACTGTGCGCGAGCCCGGTATTGTAACAGTGCCAACTGCTATGGCGAAGTTGACTCCTGGGGCCAGGGAAC14070.1008250166429354No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14060.10075335707176061No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCT14010.10039505921588664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27750.040.0709271
GGTATCA12150.038.534741
TATCAAC31300.035.289712
ATCAACG32750.033.610153
TCAACGC32850.033.6077274
CAACGCA33400.033.054315
AACGCAG34650.031.961136
CGCAGAG37300.029.7826428
CGAACTA703.9005907E-529.51669329
ACGCAGA37700.029.2841877
AGAGTAC37700.029.28418711
CAGAGTA39250.028.39061710
GCAGAGT39600.028.139699
GTGGTAT6050.027.3133981
ACCGATT1000.00882785826.86086570
GAGTACG31050.024.81166612
AGTACGG31050.024.700913
TTCGGGG11850.023.80076670
TACGGGA15650.022.63642915
TAGTACT1403.8916914E-722.1097584
Produced by FastQC (version 0.11.5)