FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632557
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA77470.29427662914801084No Hit
CTCCTGCACTGGGGGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC76680.2912757444568152No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC70300.2670407516342476No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATCCGAACCACTC68550.2603932222550167No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATCCGAACCACTCA63840.2425018717543438No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT63030.23942501529881405No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA58760.2232050436134906No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG58710.22301511420265543No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT57220.2173552177597674No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG55150.2094921401511914No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT54970.2088083942721848No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATC54870.20842853545051448No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC54360.20649125545999575No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC52460.19927393784825934No Hit
GTATATTACTGTGCGAAAGTCGGCTATTACTATGATAGTGGTGGTCATAA51480.19555132139589No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT50650.1923984931760262No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC49740.18894177789882613No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT49180.18681456849747222No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC46800.1777739285417182No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG45100.17131632857332244No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG44140.1676696838852872No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG42480.16136402744555958No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC42410.16109812627039033No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAGACTCCTCATCTATGGTAACACCCATCGG41380.15718558040718586No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC41050.15593204629567375No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG40560.1540707380694891No Hit
GTCCTATGACAGCAGCCTGAGTGGTTCGGATGTCTTCGGAACTGGGACCA39570.15031013573495275No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA38520.1463216181074142No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGCCATGAGATGGGTCCGCCAGGCTCCAGGGAAGGGG38410.14590377340357683No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT36330.13800270991283378No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG36130.13724299226949313No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAGACTCCTCAT36100.13712903462299203No Hit
ATGGTAACACCCATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG35810.13602744404014802No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC35510.13488786757513702No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATCCGAACCA34990.13291260170245126No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC34730.13192496876610837No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC34630.13154510994443805No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA33810.12843026760674128No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT32920.1250495240938753No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT31890.12113697823067078No Hit
CCTCTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCACC31600.12003538764782681No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC30480.11578096884511903No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT29700.11281807003609039No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGGGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG29440.1118304370997475No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG29260.1111466912207409No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC29260.1111466912207409No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG29160.11076683239907056No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGC28790.1093613547588903No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA28720.10909545358372107No Hit
GCCCCCAGACTCCTCATCTATGGTAACACCCATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT28480.10818379241171226No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGCTTCACCGTCTCCAGAGACAATTCCAAGAACACGCTGTATCTG28080.10666435712503092No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT27970.10624651242119353No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC27920.10605658301035836No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG27820.10567672418868802No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA27330.10381541596250338No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTTCTTGT27250.10351152890516711No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27010.1025998677331583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16650.039.9077641
GTGGTAT5950.039.3465161
GTATCAA39050.039.1449971
TGGTATC6500.036.0165142
TCGTATG1800.034.60437440
TATCAAC44350.034.4663622
TCTCGTA1550.033.44914638
ATCAACG47350.032.355353
TCAACGC47750.032.012214
CAACGCA48550.031.555635
AACGCAG49150.031.1003726
CTCGTAT2050.028.68097739
AGAGTAC53200.028.5342911
CGCAGAG53600.028.514018
ACGAGAC3050.028.21876522
TTCGGGG29500.027.83067970
CAGAGTA55300.027.38846210
GCAGAGT56700.026.9550429
TTATAAG1000.008739240526.91819870
GTGTCCA27300.026.3758970
Produced by FastQC (version 0.11.5)