FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632557
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGGGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC75330.2861476503642656No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC61280.2327774859195831No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA61110.23213172592274353No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC59810.22719356124102913No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT59790.22711758947669508No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG57800.21955839892545537No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT56530.2147341918902421No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT55830.21207518013854973No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA55130.20941616838685734No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT50590.192170577883024No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC49750.18897976378099315No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG49030.18624478026496671No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATCCGAACCACTC48590.18457340144961723No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT48480.18415555674577985No Hit
GTATATTACTGTGCGAAAGTCGGCTATTACTATGATAGTGGTGGTCATAA47520.1805089120577446No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45430.17256986268483454No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC44970.170822512105151No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATC44340.16842940152862787No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC43370.1647447709584256No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATCCGAACCACTCA43320.16455484154759042No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG42500.16143999920989366No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG42290.16064229568438596No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG40450.15365289336565172No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGCCATGAGATGGGTCCGCCAGGCTCCAGGGAAGGGG40200.1527032463114759No Hit
GTCCTATGACAGCAGCCTGAGTGGTTCGGATGTCTTCGGAACTGGGACCA39100.14852479927310216No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC39060.148372855744434No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA38920.14784105339409556No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAGACTCCTCATCTATGGTAACACCCATCGG38770.14727126516159006No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38580.1465495334004164No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG38210.14514405576023615No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG36830.1399020040211855No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC36210.13754687932682938No Hit
ATGGTAACACCCATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG35160.13355836169929083No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG35000.1329505875846183No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC34020.129227971132249No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT33900.12877214054624458No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAGACTCCTCAT33700.1280124229029039No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT33550.1274426346703984No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC33140.12588521350155002No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC32320.12277037116385325No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC31090.11809810765730808No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATCCGAACCA31070.11802213589297403No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT30890.1173383900139674No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA30660.11646471472412563No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT30590.11619881354895639No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT29980.11388167473676733No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT29720.11289404180042446No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG29030.11027301593089912No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC28880.10970322769839362No Hit
CCTCTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCACC28770.10928538299455623No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGC28610.1086776088798837No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT28470.10814580652954522No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT28450.10806983476521118No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGGGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG28360.10772796182570786No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG28030.10647442771419574No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGCTTCACCGTCTCCAGAGACAATTCCAAGAACACGCTGTATCTG27960.1062085265390265No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT27150.10313167008349677No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTTCTTGT27070.10282778302616048No Hit
GCCCCCAGACTCCTCATCTATGGTAACACCCATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT26980.1024859100866572No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA26840.10195410773631872No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26500.10066258774263957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5650.043.8650631
GGTATCA16900.042.1617241
GTATCAA41000.040.5506631
TGGTATC6700.036.9646762
TATCAAC45850.035.860622
ATCAACG48200.033.9578823
TCAACGC48800.033.533364
CAACGCA49900.032.7941475
AACGCAG50700.032.5479166
TCTGGAG30600.031.23712570
GGGCAGT7750.030.202431
CGCAGAG55500.029.609648
GTGTCCA30650.028.94255870
CAGAGTA56900.028.94152810
AGAGTAC56700.028.92234811
GCAGAGT58500.028.3850389
TTCGGGG27200.027.30439470
CGTGTTA1705.456968E-1226.62899252
GCGTGTT26550.024.51245551
AGCGTGT26900.024.18003750
Produced by FastQC (version 0.11.5)