FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086921
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1600.041.09754640
GTATCAA32050.037.8565331
CTCGTAT1400.034.57312439
GGTATCA14850.033.0046541
TATCAAC36850.032.8324132
ATCAACG37550.032.4029123
TCAACGC38350.031.6375964
CAACGCA38800.031.2706665
AACGCAG39800.030.3988576
CGTATGC2400.030.3204441
ACGCAGA42300.028.596977
GTGGTAT5700.028.2610681
TACGCGT500.007089850528.09665355
AGAGTAC43300.027.30466511
CGCAGAG44200.027.2914148
CAGAGTA43750.027.18047710
CTTATAC2850.026.457171
TCTCGTA1705.456968E-1226.41742738
GCAGAGT48200.025.0976669
TATACAC3050.024.7222753
Produced by FastQC (version 0.11.5)