FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086921
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20530.18888217266940285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32750.037.3567661
ATCAACG36950.032.7915083
TCAACGC37050.032.5152474
GGTATCA14300.032.352671
CAACGCA37350.032.3457155
CGGTCTA552.825395E-432.1328161
AACGCAG37750.031.9123176
TATCAAC38450.031.6201842
GTGGTAT5550.031.4912261
ACGCAGA39600.030.4214637
CGCAGAG40500.029.5764248
AGAGTAC40800.029.27506611
CAGAGTA41950.028.4725310
AATAACG500.00760225327.6978640
GCAGAGT43450.027.5683579
TTCGCGA658.994504E-426.36507625
TGGTATC6800.026.189482
TATCTCG1058.240404E-726.075545
AGTACGG37350.024.0989313
GAGTACG37650.023.90690612
Produced by FastQC (version 0.11.5)