FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480512
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC10090.20998435002663826No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCAAAGTGTTACTG9800.20394912093766648No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG9350.19458411028236547No Hit
TCTCTGGGCTGCAGGCTGAGGATGAGGCTCATTATTACTGCAGCTCATAT8780.18272176345231753No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG8230.17127563931806075No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCAAAGTGTTACTGCCTGCATATGAGCTG7660.15941329248801278No Hit
GTGTTACTGCCTGCATATGAGCTGCAGTAATAATGAGCCTCATCCTCAGC7240.15067261587639852No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC7230.15046450452850293No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG7090.14755094565796484No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCGGGACAGTCAGTCACCATCTCCTG7080.14734283431006925No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC6830.14214005061267979No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT6740.1402670484816196No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6630.13797782365476824No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG6610.1375616009589771No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG6480.13485615343633459No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT5580.11612613212573254No Hit
CAGTAACACTTTGCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA5400.11238012786361215No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT5050.10509623068726691No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTC200.0074562151.57397524
TGCATAG200.007462349351.56324412
CGCTAAT551.4815123E-743.8555932
GTATCAA8600.040.379621
CAATTGG350.00124489540.06591859
GGTATCA3700.039.0289541
TCGTATG557.2263592E-637.75591340
CTTATAC655.589627E-737.027471
CAAACGT400.002413561835.01309257
TATCAAC10000.034.7264752
CTCGTAT501.8299549E-434.5950939
GTTATGT400.00263855534.382651
TATACAC959.069481E-932.5730363
ATCAACG11100.031.2851143
ACGACCG450.00469529530.5655385
GACGGTA450.00470007930.5591747
ACGGTAT450.00470007930.5591748
TCAACGC11400.030.1633594
AACGCAG11300.030.125996
CAACGCA11450.030.0316415
Produced by FastQC (version 0.11.5)