FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1826291
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAAAA119130.6523056840339244No Hit
CTCCTAAACTGCTCATTTACGGGGCATCTACCCGGGAATCCGGGGTCCCT69650.3813740526564496No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58030.3177478287961776No Hit
GTTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAGAATACT43140.23621646276524386No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37430.20495090869965413No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36780.20139178258010362No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35740.1956971807888228No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34620.18956453270590504No Hit
ATATTATAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT33870.1854578487218083No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTTATTTGCAGAAACA33340.18255579203971328No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31220.17094756531133318No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30240.16558149823878013No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC29280.1603249427391363No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28850.15797044392158752No Hit
GTATAGAATACTCTGACTGGACCTGCAGTTGAAGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA28550.15632777032794884No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28340.15517789881240174No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27010.14789537921393686No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAACTGCAGGTCCAGTCAGAGTATTCTATA26810.14680026348484443No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24170.13234473586082393No Hit
TTATAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT24090.13190668956918694No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC22940.1256097741269053No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC21250.116356046216074No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21020.11509666312761767No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20870.11427532633079833No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20560.11257789695070503No Hit
GTGTATTACTGTGCGAGAGACCGGTTTGGCACCGGCTACTATGGCCTCTC20480.11213985065906802No Hit
GTTTATTACTGTCAGCAATATTATAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA19890.10890925925824525No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19790.10836170139369902No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19500.10677378358651497No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT18510.10135296072750728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)