FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276054
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29370.12903911770107387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66700.034.711951
GGTATCA33300.032.1927641
AACGCAG78750.029.4116216
TCAACGC78650.029.4055164
ATCAACG78500.029.3777663
CAACGCA80100.028.958635
TATCAAC80750.028.654012
ACGCAGA83200.027.79747
CGCAGAG86700.026.6357888
AGAGTAC86850.026.07768411
CAGAGTA88100.025.97951710
GCAGAGT92550.024.989139
GTGGTAT15800.024.2783431
AGTACGG72250.021.64009913
GAGTACG72750.021.5854212
TGGTATC18150.021.309982
GTATAGA2600.021.0768031
AATAGCG6600.019.88187462
TTAACGG5700.019.33169735
TAACGGC5750.019.17843636
Produced by FastQC (version 0.11.5)