FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235447
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCAAAGTGTTACTG31550.2553731564364963No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC29380.23780866358492106No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCGGGACAGTCAGTCACCATCTCCTG28110.2275289834367642No Hit
TCTCTGGGCTGCAGGCTGAGGATGAGGCTCATTATTACTGCAGCTCATAT27730.22445317362865422No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCAAAGTGTTACTGCCTGCATATGAGCTG27330.22121547909380168No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG26170.21182616494272924No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23220.18794816774819156No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22330.18074429740814457No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21220.17175969507392871No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20610.16682221090827853No Hit
GTGTTACTGCCTGCATATGAGCTGCAGTAATAATGAGCCTCATCCTCAGC20450.16552713309433753No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20170.16326074691994072No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC19610.15872797457114712No Hit
GTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGGTCATTTGCAGAAACA19350.15662347312349295No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17820.1442392915276819No Hit
CTATATTACTGTGCGAAAGAGACGGGCTACAATCTTTTGACTGGCTATCT17640.14278232898699825No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17580.14229667480677033No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17470.14140630880968588No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17470.14140630880968588No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17300.14003028863237355No Hit
CTCCTGCACTGGAACCACCAGTGACGTTGGTGATTATGAGTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC17220.13938274972540304No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCGGGACAGTCAGTCAC16370.13250264883884133No Hit
ATATTACTGTGCGAAAGAGACGGGCTACAATCTTTTGACTGGCTATCTCAGCTTTGACTACTGGGGCCAGGGAAG16300.13193605229524213No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16070.13007437793770188No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT15750.12748422230981984No Hit
GCCCTATATTACTGTGCGAAAGAGACGGGCTACAATCTTTTGACTGGCTA15720.1272413952197059No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15360.1243274701383386No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14600.11817585052211871No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14380.1163951185279498No Hit
CAGTAACACTTTGCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA13230.10708674674024868No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13220.10700580437687736No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13040.10554884183619369No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12890.104334706385624No Hit
GTCTTTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGT12770.10336339802516822No Hit
CAGTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGGTCATTTGCAGAAA12760.1032824556617969No Hit
GTCAGTCACCATCTCCTGCACTGGAACCACCAGTGACGTTGGTGATTATGAGTATGTCTCCTGGTACCAACAGCA12650.10239208966471244No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12370.10012570349031565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23000.033.9222261
GGTATCA11550.033.6265871
GTGGTAT5150.030.0325071
TATCAAC26000.029.988682
TCAACGC26750.029.3892174
AACGCAG27000.028.9899446
CAACGCA27050.028.9363575
ATCAACG27150.028.833283
CGACTCG500.007395141827.85535850
TGGTATC5500.027.4786132
CGCAGAG30250.026.2157848
AGAGTAC30150.025.96114511
CAGAGTA30800.025.52472510
GCCGCTT17550.025.48068270
GCAGAGT31000.025.470799
AATAATG20600.024.33933870
TTCGGGG10500.023.23044270
TACACCC8750.022.7560165
TGTTCGG17600.022.32850370
AGTACGG26050.022.00822813
Produced by FastQC (version 0.11.5)