FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062029
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15830.14905431019303614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41700.040.0074541
GGTATCA18100.038.420721
TATCAAC47500.034.8763732
TATCGGT750.00256165634.5916670
GTGGTAT7400.033.7939641
ATCAACG50200.032.9834633
TGGTATC7650.032.6618652
TCAACGC50850.032.626054
CAACGCA51650.032.120715
AACGCAG53100.031.3724276
ACGCAGA56200.029.7636557
CGCAGAG57650.028.77778
CAGAGTA57600.028.44636210
AGAGTAC57250.028.44101711
TTTCGAT1655.6742247E-627.51608870
GCAGAGT60400.027.467469
TTAGACG500.00797308227.42879328
TAACGGC658.69617E-426.5177536
CCGCTAT1851.37895295E-524.54137670
TACGGGG16900.024.08991815
Produced by FastQC (version 0.11.5)