FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058094
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39070.3692488569068533No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36540.3453379378391712No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33680.3183082032409219No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30460.2878761244275083No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG28350.26793460694418453No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26600.251395433676025No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25900.24477976436876117No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25100.23721899944617397No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24880.23513978909246247No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAACTTGTCCCAGATCCACTTCCA23980.22663392855455186No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAACTTGTCCCAGATCCACTTCCA22800.2154818002937357No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22630.2138751377476859No Hit
GTAGGAGACAGAGTCACCATCTCTTGCCAGGCGAGTCAGGGCATTAGCGACTATTTAAATTGGTATCAGCAGAAA22630.2138751377476859No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22230.21009475528639232No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21260.20092732781775532No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG21220.20054928957162596No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21210.20045478001009362No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20330.19213793859524766No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20290.1917599003491183No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19320.18259247288048133No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19080.18032424340370515No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCTCTTGCCAGGCGAGTCAGGGCATTAGCGA18820.1778669948038643No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18500.17484268883482942No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTCGCTA18430.17418112190410304No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18250.17247994979652093No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT17180.16236742671256052No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16720.15801998688207286No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16430.155279209597635No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15740.14875804985190352No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15550.14696236818278904No Hit
CCCCTAAGCTCCTCATCTACGATGCATCCAATTTGGAAACAGGGGTCCCA15000.14176434229851034No Hit
ATATAATAATCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT14940.1411972849293163No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT14870.1405357179985899No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14870.1405357179985899No Hit
CCTCATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCA14760.1394961128217342No Hit
GGGCATTAGCGACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTCATCTACGATGCATC14450.13656631641423164No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC14190.13410906781439078No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCTCTTGCCAGGCGAGTCAGG14130.13354201044519673No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT14020.13250240526834098No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13920.1315573096530176No Hit
GTCTCTGGTGCCTCCATCAACAGTGATGATTACCCGTGGAGTTGGATCCG13790.13032868535309716No Hit
ATATTACTGTCAACAATATAATAATCTCCCGCTCACTTTCGGCGGAGGGA13760.13004515666850014No Hit
ATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAACTTGTCCCAGATCCACTTCCACTGAACCTTGATGGGAC13540.12796594631478866No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13330.12598124552260953No Hit
ACCTTGATGGGACCCCTGTTTCCAAATTGGATGCATCGTAGATGAGGAGCTTAGGGGCTTTCCCTGGTTTCTG13270.12541418815341548No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12960.12248439174591293No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12850.1214447865690572No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12730.12031067183066911No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12690.11993263358453975No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT12540.11851499016155465No Hit
CATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAAGTTTTA12510.11823146147695762No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12420.11738087542316655No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12360.11681381805397253No Hit
ACATATTACTGTCAACAATATAATAATCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA12350.1167193084924402No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12140.11473460770026103No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12090.11426205989259934No Hit
TCGTAGATGAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT11980.11322245471574359No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11960.11303343559267891No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11840.11189932085429082No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11790.11142677304662914No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11720.11076520611590275No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11400.10774090014686785No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11350.10726835233920615No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11300.10679580453154444No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCT11280.10660678540847979No Hit
CACCTGCACTGTCTCTGGTGCCTCCATCAACAGTGATGATTACCCGTGGA11260.1064177662854151No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC11140.10528365154702701No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11110.10500012286242999No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10860.10263738382412149No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10810.10216483601645979No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC10810.10216483601645979No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10810.10216483601645979No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10650.10065268303194234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT309.017658E-657.61541739
CGTATGC900.050.01930241
TAGTAAT400.00879327649.4670570
ATACGAT305.4759166E-447.34411664
AGACTCG1850.046.31730725
GACTCGA1950.045.72570826
TCGACGT1900.045.1711129
CGAGACT2050.043.43349523
CTCGACG2000.042.88612728
TCGTATG1050.042.83068540
ACGAGAC2100.042.38934322
TATACAC2350.042.24463
CACGAGA2050.041.75310521
ATGTCGT503.855006E-641.38040537
CGACGTC2100.040.89041530
GTATCAA21450.040.2174261
GACGTCA2150.038.36008531
GTGGTAT2550.037.5888371
TCTCCGA2300.037.20768711
TATCAAC24250.035.43262
Produced by FastQC (version 0.11.5)