FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058094
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37950.35866378601523113No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36560.3455269569622359No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG30100.2844737802123441No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29840.28201653161250323No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26800.25328562490667184No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26500.2504503380607016No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24300.22965823452358675No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23670.2237041321470493No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23610.2231370747778553No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAACTTGTCCCAGATCCACTTCCA23600.22304256521632296No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23560.2226645269701936No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG23320.2203962974934174No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22820.21567081941680039No Hit
GTAGGAGACAGAGTCACCATCTCTTGCCAGGCGAGTCAGGGCATTAGCGACTATTTAAATTGGTATCAGCAGAAA21360.20187242343307873No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAACTTGTCCCAGATCCACTTCCA21020.19865909834097914No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20620.19487871587968555No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20260.19147637166452128No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19810.187223441395566No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19780.18693991271096896No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18650.1762603322578145No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18480.17465366971176474No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCTCTTGCCAGGCGAGTCAGGGCATTAGCGA18300.17295249760418263No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17930.169455643827486No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17240.16293448408175457No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT17240.16293448408175457No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTCGCTA17240.16293448408175457No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17130.16189487890489881No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16790.15868155381279925No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16700.15783096775900818No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16310.1541450948592469No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16270.15376705661311754No Hit
ATATAATAATCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15650.14790746379811245No Hit
CCTCATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCA14440.1364718068526993No Hit
CCCCTAAGCTCCTCATCTACGATGCATCCAATTTGGAAACAGGGGTCCCA14370.13581023992197291No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC14060.13288044351447034No Hit
GTCTCTGGTGCCTCCATCAACAGTGATGATTACCCGTGGAGTTGGATCCG14060.13288044351447034No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCTCTTGCCAGGCGAGTCAGG14050.132785933952938No Hit
GGGCATTAGCGACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTCATCTACGATGCATC13990.13221887658374398No Hit
ATATTACTGTCAACAATATAATAATCTCCCGCTCACTTTCGGCGGAGGGA13800.1304231949146295No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13780.13023417579156482No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13520.12777692719172398No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT13510.12768241763019164No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13490.12749339850712696No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13450.12711536026099762No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13390.12654830289180358No Hit
ATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAACTTGTCCCAGATCCACTTCCACTGAACCTTGATGGGAC13170.12446909253809209No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13020.12305144911510697No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12990.12276792043050995No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12840.12135027700752486No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12670.11974361446147506No Hit
ACATATTACTGTCAACAATATAATAATCTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA12630.11936557621534571No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12560.11870400928461933No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT12410.11728636586163421No Hit
ACCTTGATGGGACCCCTGTTTCCAAATTGGATGCATCGTAGATGAGGAGCTTAGGGGCTTTCCCTGGTTTCTG12400.11719185630010187No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT12330.11653028936937551No Hit
CATCTACGATGCATCCAATTTGGAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAAGTTTTA12300.11624676068477849No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12160.11492362682332571No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12070.11407304076953466No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11630.10991462006211168No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11550.10915854356985297No Hit
TTATTATATTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAACTTGTC11190.10575619935468872No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC11020.10414953680863892No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11000.10396051768557427No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10960.1035824794394449No Hit
TCGTAGATGAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT10900.10301542207025086No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCT10840.10244836470105681No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC10830.10235385513952447No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10810.10216483601645979No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10790.1019758168933951No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGAGATT10690.10103072127807171No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10590.1000856256627483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20400.042.1262971
GTGGTAT3150.038.0423431
TATCAAC22700.037.5190432
TCAACGC24600.034.8778764
ATCAACG24550.034.812963
CAACGCA25650.033.3168565
AACGCAG25950.032.931696
CGCAGAG27850.030.8077458
AGAGTAC27850.030.68500511
TAAACCG450.004835950730.3849755
GCAGAGT28700.029.895329
CAGAGTA28600.029.88032710
TAACGGC703.9003142E-529.51599536
TAGGCAA1603.3277174E-629.46875870
ACGCAGA30200.028.2972667
GAGTACT10150.027.615912
TTAACGG809.727749E-525.80929635
GTTTAAC809.816179E-525.77496133
AGTACTT10850.025.20412813
TGTTAGA10700.024.55073770
Produced by FastQC (version 0.11.5)