FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244424
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50100.4025958997897823No Hit
GTATGATACTTATCTTCGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT44510.35767551895495425No Hit
GTATCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAGTCTGTC36610.29419233315975907No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33060.26566507878343715No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC32090.25787030786934356No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31480.25296844162439813No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30820.24766478306429318No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28670.23038771351243628No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28530.22926269502998978No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28280.22725373345419247No Hit
GGTATCAACAGAGACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCAT27410.2202625471704178No Hit
GTATCAACAGAGACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGT27330.21961967946616265No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC26840.2156821147775999No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25540.20523551458345385No Hit
CACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATGATACTTATCTTCGGACGTT25110.20178010067308247No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG24660.1981639698366473No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24510.1969585928911689No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA23320.1873959357903737No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT22500.1808065418217585No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22080.177431486374419No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22030.17702969405925956No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21880.17582431711378116No Hit
GACTTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATGATACTTAT21220.17052065855367624No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTAATAGGTGGTTGGCCTGGTATCAACAGAGA20690.16626166001298592No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19970.16047585067468967No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19610.1575829460055415No Hit
CTGTTGATACCAGGCCAACCACCTATTAATATTCTGACTGGCCCGGCAAG19340.15541326750368042No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18760.15075247664783065No Hit
ATCTGGGACAGACTTCACTCTCACCATCACCAGCCTGCAGCCTGATGATT18310.14713634581139548No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17010.13668974561724942No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16670.13395755787416508No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16660.13387719941113319No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16350.1313860870571445No Hit
GATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCA16290.13090393627895316No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15970.12833246546193258No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15960.1282521069989007No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15690.1260824284970396No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15550.12495741001459308No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15360.12343059921698714No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14960.12021626069571142No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC14510.11660012985927626No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14390.11563582830289355No Hit
GAATATTAATAGGTGGTTGGCCTGGTATCAACAGAGACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGT14220.11426973443135137No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14200.11410901750528758No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14170.1138679421161919No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14110.11338579133800056No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT13870.11145718822523512No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13840.11121611283613946No Hit
TCACCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTAT13620.10944822664943782No Hit
TCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCACT13590.10920715126034213No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13540.10880535894518266No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13280.10671603890635346No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13190.10599281273906644No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13120.10543030349784317No Hit
GACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTCTAATTTAGAA13100.10526958657177939No Hit
GTTGATACCAGGCCAACCACCTATTAATATTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATG12740.10237668190263127No Hit
CCTTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTCTCTGTTGATACCAGGCCAACCACCTATTAATATTCTGA12580.10109094649412098No Hit
CCTTGGTCCCTTGGCCGAACGTCCGAAGATAAGTATCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGG12560.1009302295680572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)