FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244424
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50310.404283427513452No Hit
GTATGATACTTATCTTCGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT43610.3504432572820839No Hit
GTATCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAGTCTGTC38550.30978187498794624No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31470.25288808316136624No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30420.2444504445430175No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29090.23376276895977574No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC28750.23103058121669143No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28240.2269322996020649No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28060.22548584726749082No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27690.2225125841353108No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26570.21351243627573882No Hit
GGTATCAACAGAGACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCAT25270.2030658360815928No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24900.20009257294941277No Hit
GTATCAACAGAGACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGT24530.1971193098172327No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24080.19350317898079752No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22910.1841012388060661No Hit
CACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATGATACTTATCTTCGGACGTT22870.18377980495393853No Hit
GACTTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATGATACTTAT22690.18233335261936445No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22110.17767256176351467No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA20610.16561879230873078No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20340.16344911380686966No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG20210.16240445378745508No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19740.15862760602495612No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTAATAGGTGGTTGGCCTGGTATCAACAGAGA19320.15525255057761664No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19060.15316323053878741No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT18990.15260072129756416No Hit
CTGTTGATACCAGGCCAACCACCTATTAATATTCTGACTGGCCCGGCAAG18370.1476184965895868No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17480.14046659337974837No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17300.13902014104517432No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16890.13572544406086673No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16840.13532365174570724No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16760.13468078404145212No Hit
ATCTGGGACAGACTTCACTCTCACCATCACCAGCCTGCAGCCTGATGATT16470.13235038861352724No Hit
GTCCCAGATCCACTGCCGCTGAACCTCGATGGGACCCCACTTTCTAAATT15860.12744852236858176No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15830.1272074469794861No Hit
GAGTGAAGTCTGTCCCAGATCCACTGCCGCTGAACCTCGATGGGACCCCA15640.12568063618188013No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15610.12543956079278445No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15530.12479669308852932No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15470.12431454231033795No Hit
GATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCA15390.1236716746060828No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15360.12343059921698714No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15350.12335024075395525No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14780.11876980836113735No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14350.11531439445076597No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14300.11491260213560653No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14280.11475188520954274No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13740.11041252820582052No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC13600.10928750972337403No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13380.10751962353667238No Hit
GAATATTAATAGGTGGTTGGCCTGGTATCAACAGAGACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGGCGT13290.10679639736938536No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13030.10470707733055615No Hit
GTTGATACCAGGCCAACCACCTATTAATATTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATG12930.10390349270023722No Hit
CCTTGGTCCCTTGGCCGAACGTCCGAAGATAAGTATCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGG12910.10374277577417344No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12860.10334098345901398No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12800.1028588326808226No Hit
TCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCACT12720.10221596497656747No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12640.10157309727231233No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAT150.002417839868.5476159
TAGCGAC200.007553067551.4107137
GTGGTAT4950.043.6914371
ATCAACG31950.032.6136933
TCAACGC31950.032.6123854
CAACGCA32650.032.1218535
AACGCAG32950.031.7253766
AGAGTAC35200.029.88981211
CAGAGTA35900.029.40482910
CGCAGAG35550.029.3086858
GCAGAGT36500.029.2031639
ACGCAGA36650.028.4290267
ACGGGTA854.6567257E-628.22889116
TACGGGT3000.027.42014915
TGTTAGA9000.026.1901470
GGGAACG3900.025.90233670
GTATCAA50200.025.575781
GAGTACT14750.025.09338212
AGTACTT14700.024.71246113
AGTACGG27350.024.43457213
Produced by FastQC (version 0.11.5)