FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63643
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT1330.20897820655845892No Hit
GTATAATAACTGGCCTCCGAATACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT1240.1948368241597662No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC1100.1728391182062442No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA940.1476988828307905No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG940.1476988828307905No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG900.1414138239869271No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC890.13984255927596123No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC790.12412991216630266No Hit
GTCTATGTCTTCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTA780.1225586474553368No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTTTGGCTCCCAGATATCA780.1225586474553368No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC730.11470232390050751No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT720.11313105918954165No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGACTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG690.1084172650566441No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT660.10370347092374653No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG650.10213220621278067No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.10213220621278067No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG640.10056094150181481No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCT200.0075298651.39351336
ACCGTCC200.007553617551.35262335
GTACCGT200.00791671650.74705513
GTACATA200.00791671650.7470557
ACGGGGG200.00791671650.74705516
CGTACCG200.00791671650.74705512
CGTGTGG200.00791671650.74705517
AGTTAAG200.00791671650.7470555
ACCGTGT200.00791671650.74705515
TACCGTG200.00791671650.74705514
GGTATCA507.94098E-847.3639141
GTATCAA2250.046.612111
TACAACA306.900735E-445.108492
GATATCA504.5683235E-543.6888369
TATCAAC2450.042.8070372
CAACGCA2550.041.128335
ATCAACG2550.041.128333
TCAACGC2600.040.33744
AGATATC508.869191E-539.05036568
AACGCAG2700.038.8434226
Produced by FastQC (version 0.11.5)