FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3441394
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT106270.3087992830812165No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT83640.24304104673861815No Hit
GAGTTTACACTCAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGATTTATTACTGCATGCAAACTTTACAACCT77540.2253156714982359No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG74320.21595899801068985No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC70190.20395804723318517No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG69700.2025342056155151No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT68070.19779775288734738No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC64880.1885282533763934No Hit
GTAATAAACAGCCGTGTCGTCGACTCGCAGGCTGTCCATTTGGAGATACA63810.18541904821127717No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT63140.18347216273405487No Hit
GTAATGAATACACCTATTTCCATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGTTT63060.18323969879647609No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC63000.18306535084329198No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG57820.16801331088506574No Hit
GTTTATTACTGTGCGAGAGAGAGGACTCGAAGGTCACCACTACCAACTGGGATTGACTACTGGGGCCGGGGAACC57790.16792613690847372No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC55430.1610684507498996No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT55410.16101033476550491No Hit
GTGTAAACTCTGTGCCAGAGCCACTGCCACTGAACCTGTCAGGGACCCCG55210.16042917492155795No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGCTCTGGCACAGAGTTTACACTCAA53830.15641917199832395No Hit
GGTCTAGTCAGAGCCTCCTGCATGGTAATGAATACACCTATTTCCATTGGTACCTGCAGAAGCCAGGGCAGTCTC53150.15444322852890427No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACGAAGAC52290.15194424119993236No Hit
GGCCAAAAGTGTACGGAGGTTGTAAAGTTTGCATGCAGTAATAAATCCCA51660.15011358769149943No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT49290.14322684354072795No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC47570.13822886888278413No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA47080.1368050272651141No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAAACCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA46830.1360785774601804No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCGGGAGAGCCGGCCTCCATCTCCT46370.1347419098191024No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT46080.1338992280453793No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC46040.1337829960765899No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT45950.13352147414681376No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGTTTCTAATCGGGCC45260.13151647268519676No Hit
GTTCAGTGGCAGTGGCTCTGGCACAGAGTTTACACTCAAAATCAGCAGAG45230.1314292987086047No Hit
CCCCGGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG44490.12927900728600097No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC41790.12143334939271702No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC40410.11742334646948302No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT39720.11541834500786599No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG39360.11437225728876148No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT39070.11352957551503838No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG38470.1117860959831975No Hit
CTTTACAACCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA37300.1083863108961078No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG36790.10690435329404305No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG36770.10684623730964837No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36240.1053061637231889No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA35370.10277811840201964No Hit
CTCCACAACTCCTGATCTATTTGGTTTCTAATCGGGCCTCCGGGGTCCCT35140.10210978458148065No Hit
CTCCCTGCCCGTCACCCCGGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA34980.10164485670632306No Hit
GTACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA34950.10155768272973104No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG34930.10149956674533633No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)