FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3441394
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT103600.3010407991645246No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT89810.2609698279243818No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT78640.22851205063994418No Hit
GAGTTTACACTCAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGATTTATTACTGCATGCAAACTTTACAACCT76670.2227876261770666No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG68400.19875666662985988No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC66830.19419456185487627No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG66460.19311941614357436No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT65680.19085289275218126No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC61120.1776024483101906No Hit
GTAATAAACAGCCGTGTCGTCGACTCGCAGGCTGTCCATTTGGAGATACA58500.16998925435448542No Hit
GTTTATTACTGTGCGAGAGAGAGGACTCGAAGGTCACCACTACCAACTGGGATTGACTACTGGGGCCGGGGAACC57540.16719968710354No Hit
GTGTAAACTCTGTGCCAGAGCCACTGCCACTGAACCTGTCAGGGACCCCG56690.16472975776676543No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56560.16435200386819993No Hit
GTAATGAATACACCTATTTCCATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGTTT56220.1633640321334901No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC51190.14874786205822407No Hit
GGTCTAGTCAGAGCCTCCTGCATGGTAATGAATACACCTATTTCCATTGGTACCTGCAGAAGCCAGGGCAGTCTC50880.1478470643001063No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT50710.1473530784327514No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACGAAGAC50640.14714967248736993No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT50070.14549336693212112No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGCTCTGGCACAGAGTTTACACTCAA49790.14467974315059537No Hit
GGCCAAAAGTGTACGGAGGTTGTAAAGTTTGCATGCAGTAATAAATCCCA49770.14462162716620067No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC48310.14037916030538786No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46340.13465473584251034No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT45360.13180705260717024No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAAACCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA44290.128697847442054No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC43960.1277389336995415No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA43890.1275355277541601No Hit
GTTCAGTGGCAGTGGCTCTGGCACAGAGTTTACACTCAAAATCAGCAGAG43870.1274774117697654No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCGGGAGAGCCGGCCTCCATCTCCT43800.12727400582438395No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG43190.12550146830034573No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGTTTCTAATCGGGCC42040.12215979919765073No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT41210.11974798584527084No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT39750.11550551898445804No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC39470.1146918952029323No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC39310.11422696732777474No Hit
CTCCCTGCCCGTCACCCCGGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA37720.1096067465683964No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT37430.1087640647946733No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGA37120.10786326703655554No Hit
CCCCGGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG37100.10780515105216083No Hit
GGGTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT35480.10309775631619046No Hit
CTCCACAACTCCTGATCTATTTGGTTTCTAATCGGGCCTCCGGGGTCCCT35410.10289435037080905No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA35360.1027490604098223No Hit
GTACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA35000.10170297269071776No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT34920.101470508753139No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG34820.1011799288311655No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC34730.10091840690138938No Hit
CTTTACAACCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA34610.1005697109950212No Hit
CTCCAGCCCCTTGCCTGGAGACTGGCGGACCCAGTGCATGGCATAGAAGC34490.10022101508865304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8950.045.3180121
GGTATCA26900.039.228161
CCTTTGT34900.038.5031170
TGGTATC10500.037.2883952
GTATCAA57650.036.8469051
TATCAAC65450.032.0619352
TCAACGC69700.030.0415064
ATCAACG70100.029.9233973
CAACGCA70450.029.8191345
AACGCAG72350.029.1783836
ACGCAGA77200.027.2118937
AGAGTAC77800.026.78181611
CGCAGAG78300.026.7423158
CAGAGTA79850.026.18022210
TGTTAGA22000.024.12657470
TACACCC31200.022.9940935
TCTGGAG33950.022.956770
TTCGGGG27550.022.92437470
AGTACTT28250.021.99301313
AAGGCTT38700.021.77627869
Produced by FastQC (version 0.11.5)