FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715468
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18200.036.559071
AGACGTA851.2658711E-732.2959825
GGTATCA8400.030.6229361
TATCAAC21900.030.3803022
CTCGTAT802.6803755E-630.30062539
ATCAACG22050.030.1736343
CAACGCA22150.030.0374095
TCAACGC22250.029.7482744
AACGCAG22800.029.181086
TCGTATG658.411149E-426.66802640
ACGCAGA25050.026.421277
CTACCGT658.959875E-426.3809916
AGAGTAC25200.025.85575311
CAGAGTA25500.025.82053210
CGCAGAG26100.025.3583458
CTTATAC1501.1150405E-925.1516381
CGTATGC700.001284864424.79996141
GCAGAGT26750.024.7421639
ACGTAGA1151.7711372E-623.8809627
GACGTAG1202.567198E-622.88271726
Produced by FastQC (version 0.11.5)