FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952210
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAGTACCTTACCATACACTTTCGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCAT23600.24784448808561135No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22740.2388128669096103No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21060.221169699961143No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA20790.2183341909872822No Hit
GTATTACTGTCATCAGAGTAGTACCTTACCATACACTTTCGGCCAGGGGA19120.2007960428896987No Hit
GTACTACTCTGATGACAGTAATACACTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTC18070.18976906354690667No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16510.17338612280904422No Hit
CAGTAATACACTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA15000.15752827632560046No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14290.15007193791285536No Hit
GATCAGGACTGAGCACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA14270.1498619002110879No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14240.1495468436584367No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14220.14933680595666923No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13740.14429590111425No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13520.14198548639480787No Hit
GTGTATTACTGTCATCAGAGTAGTACCTTACCATACACTTTCGGCCAGGGGACCAAGCTGGAGCTCAAACGAA13260.1392549962718308No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12580.13211371441173692No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12390.13011835624494597No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11770.1236071874901545No Hit
GGATACACTGGTACCAGCAGAAACCACATCAGTCTCCAAAGCTCCTCATC11000.11552073597210699No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10880.11426050976150219No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC10520.11047983112968777No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCCCCTGGCCGAAAGTG10430.10953466147173418No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC10280.10795937870847817No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG10040.10543892628726856No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10010.10512386973461736No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT9760.10249839846252402No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC9760.10249839846252402No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9620.10102813455015176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)