FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005757882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952210
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24960.26212705180579915No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24340.2556158830510077No Hit
GAGTAGTACCTTACCATACACTTTCGGCCAGGGGACCAAGCTGGAGCTCAAACGAACTGTGGCTGCACCAT22920.2407032062255175No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA21930.23030633998802783No Hit
GTATTACTGTCATCAGAGTAGTACCTTACCATACACTTTCGGCCAGGGGA18270.19186944056458136No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17640.18525325295890613No Hit
GTACTACTCTGATGACAGTAATACACTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTC17420.18294283823946397No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15960.1676100860104389No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14820.15563793700969325No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14020.14723642893899455No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14010.14713141008811081No Hit
CAGTAATACACTGCAGCATCTTCAGGTTCCAGGCTATTGATGGTGAGGGTGAAATCTGTCCCAGATCCACTGCCA13640.1432457126054127No Hit
GATCAGGACTGAGCACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA13570.14251058064922653No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13060.13715461925415612No Hit
GTGTATTACTGTCATCAGAGTAGTACCTTACCATACACTTTCGGCCAGGGGACCAAGCTGGAGCTCAAACGAA12690.133268921771458No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12060.12665273416578277No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11910.12507745140252674No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11550.12129677277071234No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10950.11499564171768832No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10690.11226515159471125No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10360.10879952951554804No Hit
GGATACACTGGTACCAGCAGAAACCACATCAGTCTCCAAAGCTCCTCATC10330.10848447296289683No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9830.10323353041871017No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCACGGCG9590.10071307799750055No Hit
GTGCAGCCACAGTTCGTTTGAGCTCCAGCTTGGTCCCCTGGCCGAAAGTG9560.10039802144484936No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT9550.10029300259396562No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9530.10008296489219815No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)