FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603566
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64390.4015425620149093No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGCA44160.2753862329333498No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT39860.24857099738956798No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38130.23778254215916275No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37480.23372907632114923No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT37280.2324818560632989No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC36740.22911436136710306No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36690.2288025563026405No Hit
GTTTATTACTGCATGCAAAATTCACACTGGCCGGCGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA35160.21926132133008558No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT34670.2162056316983523No Hit
GGCCAGGCCAGTCTCCAAGGCGCCTCATTTATAAGGTTTCTAACCGGGAC33490.2088470321770354No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33240.2072880068547225No Hit
GTAATAAACCGCAACATCCTCAGCCTCCACCCTGCTGATTGTCAGTGTGAAATCAGTGCCTGACCCACTGCCG32180.20067773948811585No Hit
GTGGTGGAAACACCTACTTGAGTTGGTTTCAGCAGAGGCCAGGCCAGTCTCCAAGGCGCCTCATTTATAAGGTTT31680.19755968884349007No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT31620.19718552276613496No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACGCCTGG30930.1928826128765514No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30640.19107414350266844No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30540.1904505333737433No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC30290.18889150805143037No Hit
CCATAGGACTGTGGTACAGTAATACACGGCTGTGTCCTCGGTTTTCAGGC28410.1771676376276374No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAC27410.17093153633838581No Hit
GTGGTACAGTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTG27360.17061973127392324No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG26360.16438362998467165No Hit
TTATTACTGCATGCAAAATTCACACTGGCCGGCGACTTTTGGCCAGGGGA25370.1582098897083126No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25080.15640142033442964No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC24690.15396934083162153No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23950.14935462587757536No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23660.14754615650369238No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC23620.14729671245212234No Hit
GTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACA23390.14586240915559445No Hit
CAGTAATAAACCGCAACATCCTCAGCCTCCACCCTGCTGATTGTCAGTGTGAAATCAGTGCCTGACCCACTGCCG22780.142058387369151No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAACTTCGGCACCCAGAC22660.14131005521444082No Hit
CTCCAAGGCGCCTCATTTATAAGGTTTCTAACCGGGACTCTGGGGTCCCA22550.14062408407262314No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22550.14062408407262314No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACGCCTGGATGTACTGGGTCCGCCAGGCTCCAGGGAAGGGG22340.13931450280188032No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21700.1353233979767593No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATGAGGCGCCTTGGAGACTGGCCTGGCCTCTGCTGAAA21170.13201826429345595No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20950.1306463220098206No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20800.12971090681643288No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGCATA20570.12827660351990502No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20570.12827660351990502No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGGGCTTGCTGGCCGTGGCACTCATTTA19960.12447258173346155No Hit
GATTTCACACTGACAATCAGCAGGGTGGAGGCTGAGGATGTTGCGGTTTATTACTGCATGCAAAATTCACACTGG19950.12441022072056902No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA19830.12366188856585884No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19730.12303827843693367No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19670.12266411235957858No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA19410.12104272602437317No Hit
GTGTGAGGTTCACCTGGTGGAGTCTGGGGGAGGCTTGGTAAAGCCTGGGGAGTCCCTTAGACTCTCCTGTGCAGC19300.12035675488255551No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT19040.1187353685473501No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18960.11823648044420998No Hit
GGGTGGAGGCTGAGGATGTTGCGGTTTATTACTGCATGCAAAATTCACACTGGCCGGCGACTTTTGGCCAGGGGA18820.11736342626371475No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18410.1148066247351216No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGCTGTGATGACTCAGT18330.11430773663198147No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18280.1139959315675189No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18260.11387120954173385No Hit
GTCCTATGGTTCGGGGACTTAGAGATTGACTACTGGGGCCAGGGAACCCT17840.11125204700024818No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAA17790.11094024193578561No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17680.11025427079396795No Hit
CCGCTGGTCAGAGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA17640.11000482674239788No Hit
GACAATCAGCAGGGTGGAGGCTGAGGATGTTGCGGTTTATTACTGCATGC17450.10881996749744008No Hit
GGCCTGGCCTCTGCTGAAACCAACTCAAGTAGGTGTTTCCACCACTGTAT17320.10800927432983737No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17290.10782219129115982No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGCTGTGATGACTCAGTCTCCACTCTCC17280.10775983027826733No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACAAGACAGTTGAGCGCAAATGTTGTGT17240.10751038622669724No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTT17130.10682441508487958No Hit
GCAGTGGGTCAGGCACTGATTTCACACTGACAATCAGCAGGGTGGAGGCT16980.10588899989149184No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG16880.10526538976256666No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC16730.10432997456917895No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16700.1041428915305014No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16690.10408053051760888No Hit
GTGTGAATTTTGCATGCAGTAATAAACCGCAACATCCTCAGCCTCCACCC16330.10183553405347832No Hit
GTCTCCAAGGCGCCTCATTTATAAGGTTTCTAACCGGGACTCTGGGGTCC16290.10158609000190824No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16260.1013990069632307No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16180.10090011886009058No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA16070.10021414771827289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4600.056.1102181
TGGTATC4850.053.181422
CCTTTGT16100.040.18022570
GGTATCA12350.040.126761
GTATCAA28750.036.9878581
TATCAAC32050.032.727452
ATCAACG33500.031.1975273
TCAACGC33850.030.9745854
CAACGCA34500.030.1917215
AACGCAG35000.029.563976
TCTTGAG14400.028.5452870
ACGCAGA37700.027.446667
CGCAGAG38650.026.8609738
GCAGAGT39650.026.3569249
AGAGTAC39050.026.32173211
CAGAGTA39550.026.16280710
CTTATAG700.001358981224.5647532
GTATAGG1001.5945869E-524.0899891
TACACTA1001.6081263E-524.06375
GAATCAT18550.023.97546270
Produced by FastQC (version 0.11.5)