FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3024141
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT99440.32882064692089424No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA72100.23841480936239415No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGTCTGAAGATT69810.2308424111177356No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGCAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG67890.22449350079906988No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC61270.20260298709617047No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG58660.19397243713173426No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT58510.19347642851308852No Hit
CTACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG58050.1919553354159082No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC56980.18841714060290177No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC56920.1882187371554435No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT54210.1792575147785768No Hit
GAGTATTAGCAGCTCCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGAGGCATC53300.1762483958254592No Hit
GTGCAGCCACAGTTCGCTTGAGATCCACTTTGGTCCCAGGGCCGAAAGTG52540.17373528549098735No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG51360.16983335102430738No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT49490.16364977691185695No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC47410.15677179073330244No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT47160.1559451097022262No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT46450.15359733557396962No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC46050.15227464592424758No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTA44900.14847191318129677No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTCCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT44210.1461902735355263No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44010.14552892871066528No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG43820.1449006511270473No Hit
GGTCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG43320.1432472890648948No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT41260.13643543736882638No Hit
ATCCTACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG39400.130284930497619No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC36470.12059622881340519No Hit
GCGTAGCAACTGGCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATCTCAAGCGAACTGTGGCTGCACCAT36360.12023248915973164No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGTCTGAAGATTCTGCAGTTTACTACTGTCAGCAGCGTAGCAACTGG35550.11755404261904455No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT35000.11573534435067676No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC34630.1145118564246839No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG33470.11067605644049004No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG33030.10922109782579582No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGAGATCCACTTTGGTCC33020.10918803058455276No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA33000.10912189610206666No Hit
GCCTAGAGTCTGAAGATTCTGCAGTTTACTACTGTCAGCAGCGTAGCAACTGGCCTTTCACTTTCGGCCCTGGGA32460.10733626507494194No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32270.10670798749132399No Hit
GTTTACTACTGTCAGCAGCGTAGCAACTGGCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATCTCAAGCGAA32250.10664185300883787No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA31500.1041618099156091No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG31250.10333512888453283No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA30900.10217777544102606No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA30450.1006897495850888No Hit
GTCTTTGTCTCCAGGAGCAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTCCTTAGCCTGGTA30280.10012760648395694No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATGGCTAC30250.10002840476022777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22150.041.4111981
GTATCAA49550.038.410181
TATCAAC57900.032.8115542
CGCTAAT2100.032.7980432
ATCAACG59300.031.979513
TCGTATG1850.031.77365740
TCAACGC60000.031.6064174
CAACGCA61050.031.2321515
AACGCAG63550.030.0575686
CGTATGC1850.029.9190641
TGCACAT19950.027.83239670
CGCAGAG69750.027.3347198
CTCGTAT2200.026.70275739
AGAGTAC71000.026.61111511
TCGCTAA2800.025.8190431
GCAGAGT74700.025.5693749
CTTATAC3800.025.3136441
TTCGGGG26350.024.9270470
TGTTAGA17150.024.47976370
AATCGAG2501.5174373E-724.37710670
Produced by FastQC (version 0.11.5)