FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3024141
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT100790.33328472448870605No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA67520.22327001287307702No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGTCTGAAGATT66630.22032702840244553No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT66610.22026089391995943No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT63470.20987778016964154No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGAGCAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG60490.20002374227921252No Hit
GTGCAGCCACAGTTCGCTTGAGATCCACTTTGGTCCCAGGGCCGAAAGTG59380.1963532785012339No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG55500.1835231888989303No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC54910.18157222166559034No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC54890.18150608718310424No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT54220.17929058201981984No Hit
CTACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG51780.17122217515651553No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC51420.17003175447176572No Hit
GAGTATTAGCAGCTCCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGAGGCATC48690.1610043976124129No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT47990.15868969072539937No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTA46630.15419254591634451No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46190.1527375873016503No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42640.14099871666036734No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCTCCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT42560.14073417873042296No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC41000.13557568909650708No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC40980.13550955461402098No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40610.1342860666880281No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT40010.13230203221344508No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT39630.13104547704620917No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT38620.12770568568066107No Hit
GGTCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG37060.12254719604674517No Hit
ATCCTACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG36980.12228265811680078No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG36750.1215221115682106No Hit
GCGTAGCAACTGGCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATCTCAAGCGAACTGTGGCTGCACCAT35850.11854605985633607No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGTCTGAAGATTCTGCAGTTTACTACTGTCAGCAGCGTAGCAACTGG34760.11494173056084354No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA34550.1142473184947395No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA34300.11342063746366322No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG33360.11031231678681648No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33280.11004777885687209No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA32330.10690639093878229No Hit
GTTTACTACTGTCAGCAGCGTAGCAACTGGCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATCTCAAGCGAA32240.10660878576759483No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGAGATCCACTTTGGTCC32160.10634424783765044No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATGGCTAC31420.10389727198566469No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT30850.1020124392348108No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT30630.1012849599274637No Hit
GCCTAGAGTCTGAAGATTCTGCAGTTTACTACTGTCAGCAGCGTAGCAACTGGCCTTTCACTTTCGGCCCTGGGA30620.10125189268622066No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC30550.1010204219975193No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC30310.10022680820768609No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA30280.10012760648395694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23800.039.9951021
GTATCAA55400.039.5124241
TATCAAC62500.034.9456862
ATCAACG65500.033.4322783
TCAACGC66000.033.1198774
CAACGCA67350.032.4565
AACGCAG68600.031.814586
TTCGGGG23800.029.56354370
CGCAGAG74750.029.1965778
AGAGTAC74550.028.81460811
GCAGAGT78650.027.7488149
ACGCAGA83300.026.2414387
TGTTAGA16850.024.15379770
AGTACGG62950.023.93066813
GAGTACG63550.023.81272512
TGCACAT21400.023.20876570
CGATTCA18550.021.56837370
AGTACTT29150.021.42490213
GAGTACT30250.021.21300912
TACACCC22000.021.0573795
Produced by FastQC (version 0.11.5)