FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897995
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19880.2213820789648049No Hit
CCCCTACACACTTTCGGCCCTGGGACCCAAGTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC15290.17026820862031525No Hit
GTAATAAACAGCCGTGTCTGCGGCGGTCACAGACCTCAACTTCAGGGAGA14650.16314122016269578No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14530.16180490982689213No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14170.15779597881948118No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13900.15478928056392297No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13720.1527848150602175No Hit
GTCCCAGACCCACTGCCCCTGAACCTGGCTGGGATGCCAGTGGCCCTGTT13450.14977811680465927No Hit
GTTTATTACTGTGCGAGACCAATGGTGGTGGCTGCTGTAACGACCGGTTACTTCGATCTCTGGGGCCGTGGCACC13300.1481077288849047No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13290.1479963696902544No Hit
GTGTAGGGGAGGCCAGTCGGTTCGCTGCTGACAGTAATAAACTGCAATAT12870.1433192835149416No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12790.1424284099577392No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTGGCAC12710.14153753640053673No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12150.1353014215001197No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11750.13084705371410754No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11700.130290257740856No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11590.12906530659970267No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10980.12227239572603411No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10960.1220496773367335No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10890.12127016297418136No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCACCCTTGAGCCTGAAGATA10670.11882026069187468No Hit
GTGCAGCCACAGTTCGTTTAATGTCCACTTGGGTCCCAGGGCCGAAAGTGTGTAGGGGAGGCCAGTCGGTTCG10260.1142545337112122No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9990.11124783545565398No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTGGCACCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT9600.10690482686429212No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9600.10690482686429212No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9540.10623667169639028No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9290.1034526918301327No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9120.10155958552107751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGC1400.049.1337728
ACTAGCG1550.044.37146427
TCGTATG651.1041266E-842.60621640
CGAGACT1650.041.64745723
CACGGAT5450.040.84924370
TAGCGCT1700.040.4698729
ACGAGAC1750.039.26541522
GTATCAA14600.037.874431
CTTATAC2150.036.7419741
GGTATCA7000.035.817721
TATACAC2200.034.347673
AGACTAG2050.033.53420325
TATCAAC16700.033.5249752
TTATACA2400.032.916522
CGTATGC958.463758E-932.81214541
CCCACGA2250.032.06139819
ATCTCCG2250.032.0578310
TATAGTG450.004723294630.5312652
TCAACGC18350.030.5104664
ATCAACG18500.030.448753
Produced by FastQC (version 0.11.5)