FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897995
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18750.20879848996932054No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17160.19109237801992215No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15060.16770694714335824No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14800.16481160808245035No Hit
GTAATAAACAGCCGTGTCTGCGGCGGTCACAGACCTCAACTTCAGGGAGA14480.1612481138536406No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14450.1609140362696897No Hit
CCCCTACACACTTTCGGCCCTGGGACCCAAGTGGACATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC14370.16002316271248726No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13140.14632598177049982No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12970.14443287546144465No Hit
GTTTATTACTGTGCGAGACCAATGGTGGTGGCTGCTGTAACGACCGGTTACTTCGATCTCTGGGGCCGTGGCACC12560.1398671484807822No Hit
GTCCCAGACCCACTGCCCCTGAACCTGGCTGGGATGCCAGTGGCCCTGTT12350.1375286053931258No Hit
GTGTAGGGGAGGCCAGTCGGTTCGCTGCTGACAGTAATAAACTGCAATAT12310.13708316861452458No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTGGCAC12190.13574685827872093No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11330.12616996753879475No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11170.12438822042438989No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11090.12349734686718747No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11040.12294055089393595No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCACCCTTGAGCCTGAAGATA10570.11770666874537164No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10470.11659307679886859No Hit
GTGCAGCCACAGTTCGTTTAATGTCCACTTGGGTCCCAGGGCCGAAAGTGTGTAGGGGAGGCCAGTCGGTTCG10210.11369773773796067No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9750.10857521478404668No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTGGCACCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT9740.10846385558939639No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9730.10835249639474606No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9420.10490036136058664No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9330.10389812860873389No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8980.10000055679597326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16200.039.0002631
CTATACA557.68915E-637.4212074
GGTATCA7250.036.468461
TAATCGT400.002581559834.53933338
TATCAAC18400.034.316152
TCAACGC18800.033.5729264
ATCAACG19050.033.1341863
CAACGCA19400.032.7114075
CACGGAT6000.032.47339670
ACGGGTA751.7443199E-632.01770416
AACGCAG19850.031.969846
TACTACG450.004749728430.49644718
CGCAGAG21400.029.6542668
AGAGTAC21600.029.06207311
CAGAGTA22150.028.3404410
GCAGAGT22800.027.6829419
TTAATCG500.007716409427.61288837
ACGCAGA23800.026.8080487
AGTACGG19600.025.02702313
GAGTACG20200.024.28364612
Produced by FastQC (version 0.11.5)