FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005757894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005757894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898129
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT30200.3362545914896412No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27920.31086848325797295No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25870.28804325436546424No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTCGTTACTACTGGGGCTGGGTCCG23020.2563106190758788No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21180.2358235843626027No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19560.2177860864085226No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19450.21656131802892456No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18700.20821062453166528No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18250.20320020843330971No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGCTACCA18050.20097335683404055No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17370.19340206139652544No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17030.18961641367776788No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16420.182824516299997No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15450.17202428604354164No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15310.17046548992405322No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTCCAGTCTGAAGACG15100.1681272957448206No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14870.1655664164056611No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14830.16512104608580727No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT14710.16378493512624578No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14530.16178076868690355No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14280.1589972041878171No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13850.15420947324938844No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13830.15398678808946153No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACCAGTTGAAAGCCACTGCTTGGGGTAACTGTCTCGCACAGTAAAAAACAG13740.15298470486979043No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13730.15287336228982695No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13610.15153725133026547No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAT13540.15075785327052127No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTCGTTACTACTGGG13370.1488650294111425No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT13250.147528918451581No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT13230.1473062332916541No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13070.14552475201223877No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12780.1422958171932985No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12780.1422958171932985No Hit
GGGTAACTGTCTCGCACAGTAAAAAACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGATTCAGGGAGAACTGGTT12660.14095970623373702No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12650.14084836365377357No Hit
CTATAATAACTGGCTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT12600.14029165075395628No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCC12470.13884419721443134No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12410.1381761417346506No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG12180.13561526239549107No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT12060.13427915143592958No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG11890.1323863275765508No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11840.13182961467673351No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA11720.13049350371717203No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11670.12993679081735474No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11660.12982544823739128No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA11640.12960276307746438No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC11510.1281553095379394No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11510.1281553095379394No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG11460.12759859663812215No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT11280.12559443019877992No Hit
GAGTTCACTCTCACCATCAGCAGCCTCCAGTCTGAAGACGTTGCAGTTTATTACTGTCAGCACTATAATAACTGG11100.12359026375943767No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11070.1232562360195473No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA10690.11902521798093592No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT10650.1185798476610821No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC10400.11579628316199565No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGTCC10290.11457151478239762No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10260.11423748704250726No Hit
GTAAAAAACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGATTCAGGGAGA10210.11368077414268998No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA10130.11279003350298232No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG10050.11189929286327466No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9990.1112312373834939No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9950.11078586706364008No Hit
GTTATTATAGTGCTGACAGTAATAAACTGCAACGTCTTCAGACTGGAGGCTGCTGATGGTGAGAGTGAACT9940.11067452448367662No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG9880.11000646900389587No Hit
GTAGTAGGTGATCCCATTATAAGAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGACCCAGCC9780.1088930432042613No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG9640.1073342470847729No Hit
GTGCGAGACAGTTACCCCAAGCAGTGGCTTTCAACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCT9600.10688887676491908No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA9550.10633216386510178No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG9540.10622082128513832No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA9440.10510739548550375No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA9420.10488471032557684No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9380.104439340005723No Hit
GTCTCGCACAGTAAAAAACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGA9360.10421665484579609No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC9120.10154443292667312No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCAGCTCATC9110.10143309034670966No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG8990.10009697938714818No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAG252.6404473E-454.8851363
GTGGTAT3200.049.368591
GTATCAA14450.038.50261
TATACGA400.002661145534.3261452
TATCAAC16800.033.1002122
GGTATCA7000.032.871511
ATCAACG17550.031.6644973
TCAACGC18000.031.0582734
CAACGCA18250.030.820755
AACGCAG18200.030.716976
GTATACA1052.435263E-829.4371721
CGCAGAG19600.028.3479168
AGAGTAC19850.027.98933211
GCAGAGT20050.027.5406179
GCTTGAC500.00790766727.4746951
ATACCTT1254.4183253E-927.4379846
GGTAGTC500.0079594327.437987
TGTTAGA4200.026.97702670
TTCGGGG13150.026.92573770
TACGGGC2700.026.67730315
Produced by FastQC (version 0.11.5)